| Morphological information on Globodera spp. |
Cyst nematodes belonging to the genera Globodera Skarbilovich 1959 (Behrens 1975), are economically important pests of solanaceous crops (Baldwin & Mundo-Ocampo 1991). The potato cyst nematodes, Globodera pallida (Stone 1973) Behrens 1975, and Globodera rostochiensis (Wollenweber 1923) Behrens 1975 are speculated to have originated from South America (Evans et al. 1995) and has become established in Europe. Limited introductions of G. rostochiensis has occurred to United States and Canada (Baldwin & Mundo-Ocampo 1991). Because of this, golden nematode, G. rostochiensis, has quarantine status in both the United States (U.S. Dept. Agriculture) and Canada (Canadian Food Inspection Agency). Adult female potato cyst nematodes can be discriminated morphologically, however, adult males and juveniles are difficult to identify to species (Baldwin & Mundo-Ocampo 1991). Given this problem, molecular diagnostic techniques, including RAPDs (Folkersma 1996), PCR (Shields et al. 1996), and PCR-RFLP (Thiery & Mugiery 1996) have been applied to discriminate potato cyst nematode species. |