Date sent: Wed, 09 Feb 2000 15:48:03 -0600 From: "L-Soft list server at UNIVERSITY OF NE - LINCOLN (1.8d)" Subject: File: "NEMA-L LOG9905" To: Allen Szalanski ========================================================================= Date: Thu, 6 May 1999 15:48:07 -0500 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Allen Szalanski Subject: new "shorter" SON WWW address Comments: To: nema-l@unl.edu MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT FYI, The Society of Nematologists now has a shorter and easier to remember WWW address. It is now: http://www.ianr.unl.edu/son/ Allen Szalanski ========================================================================= Date: Sun, 9 May 1999 10:00:27 +0300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: cal Subject: BT and nematodes MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dear Nematologists: I am going to start a study about the effect of Bacillus thuringiensis on Nematodes (specially on Meloidogyne spp). It is a new field for me and I don't know enough resources about it. Thus, I will be very appreciated if somebody can tell me about some resources in that field. Thanks in advance. With all of my best regards. Abd El-Nasser ========================================================================= Date: Mon, 10 May 1999 09:40:12 -0600 Reply-To: ibendezu@ppserver.tamu.edu Sender: NEMA Discussion List From: IVAN BENDEZU Subject: Re: BT and nematodes In-Reply-To: <1.5.4.16.19990509100258.12efaae6@ageri.sci.eg> MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Dear Abd El-Nasser, Sometime ago, in collaboration with Dr. Manuel Canto from CIP (International Potato Center) we did some experiments with B.t. and Globodera pallida under field conditions. As far as I remenber it was tested in crop rotations with other organic compounds, so probably he can tell you about it I do not have the data with me. His email address is m.canto@cgnet.com. Good luck. Ivan Dr. Ivan Bendezu Research Associate Plant Pathology & Microbiology Dept. College of Agriculture and Life Sciences Texas A & M University L.F. Peterson 120, College Station 77843-2132 USA ========================================================================= Date: Mon, 10 May 1999 16:33:24 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Pete Goodell Subject: Ditylenchus dipsaci management MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" Is there any recent information on the management of D. dipsaci on alliums (onion and garlic)? I am looking for host range information and associated crop rotation schemes. I have several reports from the San Joaquin Valley of fields infested with this pest on garlic. Thanks in advance for any help or direction. Pete Goodell IPM Specialist - Statewide IPM Project Kearney Ag Center Cooperative Extension, Univ of Calif. ========================================================================= Date: Tue, 11 May 1999 12:15:45 +0300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: cal Subject: in vitro culturing MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dear Nematologists: I am trying to culture Meloidogyne spp. in vitro on tomato roots. I succeded on culturing the tomato roots on MS medium and getting red of contamination by enough surface sterilization for the J2 but now after about one month of adding the J2 there is no any symptoms which can say that there was any infection. However, the J2 idividuals seems active on the media but starving. Tell now, there is no any galls. I will be very appreciated if somebody who have experience about that can tell me if there any conditions which i must avoid or keep to have success in getting galls. Thanks in advance. Abd El-Nasser ========================================================================= Date: Tue, 11 May 1999 08:00:46 -0600 Reply-To: aem@plantpath.wisc.edu Sender: NEMA Discussion List From: "Ann E. MacGuidwin" Organization: University of Wisconsin Subject: Re: in vitro culturing In-Reply-To: <1.5.4.16.19990511121821.0fafc2cc@ageri.sci.eg> Date sent: Tue, 11 May 1999 12:15:45 +0300 Send reply to: NEMA Discussion List From: cal Subject: in vitro culturing To: NEMA-L@CRCVMS.UNL.EDU Dear Abd El-Nasser: One of the easiest things to check is your timing of inoculation. We found there is a rather short window to get good infection. If you wait too long, the nematodes seem to wander aimlessly. My experience is with M. hapla, but I bet it applies to other species as well. An MacGuidwin Dear Nematologists: I am trying to culture Meloidogyne spp. in vitro on tomato roots. I succeded on culturing the tomato roots on MS medium and getting red of contamination by enough surface sterilization for the J2 but now after about one month of adding the J2 there is no any symptoms which can say that there was any infection. However, the J2 idividuals seems active on the media but starving. Tell now, there is no any galls. I will be very appreciated if somebody who have experience about that can tell me if there any conditions which i must avoid or keep to have success in getting galls. Thanks in advance. Abd El-Nasser A. E. MacGuidwin Professor and Nematologist Dept. of Plant Pathology 1630 Linden Drive University of Wisconsin Madison, WI 53706-1598 (608) 263-6131 (608) 263-2626 fax ========================================================================= Date: Mon, 10 May 1999 21:23:36 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Hassan Mojtahedi Subject: Re: in vitro culturing MIME-version: 1.0 Content-type: multipart/mixed; boundary="---- =_NextPart_000_01BE9B2B.5E4F31E0" ------ =_NextPart_000_01BE9B2B.5E4F31E0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: quoted-printable Dear colleague: We compared the reproduction efficiency of M. chitwoodi on in vitro and = pot culture sometimes ago (Plant Disease 73 (1989): 957-959). M = chitwoodi successfully infected excised roots of tomato and susceptible = potato. The problem was that we could not raise a large number of = nematode in Petri Dishes, because after 1st or 2nd generations the = nematode ceased to reproduce further. I thought that the accumulation of = byproducts of nematodes and plants inhibited the parasite to flourish = further. 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However, the J2 idividuals seems active on >the media but starving. Tell now, there is no any galls. I will be very >appreciated if somebody who have experience about that can tell me if >there any conditions which i must avoid or keep to have success in >getting galls. Thanks in advance. Abd El-Nasser Dear Dr. El-Nasser, There may be several reasons for your problem. 1. The roots: Is it too old, or is it growing vigorously? Nematodes usually prefer young growing roots. And make sure it's a susceptible variety. 2. The medium: B5, STW, White's are great media for tomato roots, too. Sometimes rich media such as MS can adversely affect nematode activities. 3. The nematode: Sometimes heavy sterilization of the J2 hurts the nematodes so much that they can't behave normally. So if you doubt about it, try some lighter procedures. Hope this helps. You should be able to see the galling begins 2-3 days after inoculation if everything is ok. Good luck! Xiang -------------------- Xiang Huang Dept. of Nematology Univ. of California Riverside, CA 92521 909-787-4586 909-787-3719 (fax) -------------------- ========================================================================= Date: Wed, 12 May 1999 08:45:16 +0930 Reply-To: ian.riley@adelaide.edu.au Sender: NEMA Discussion List From: "Ian T. Riley" Subject: Re: in vitro culturing In-Reply-To: <1.5.4.16.19990511121821.0fafc2cc@ageri.sci.eg> MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" Content-transfer-encoding: 7bit See Hutangura P., Jones M. G. K. and Heinrich T. (1998). Optimisation of culture conditions for in vitro infection of tomato with the root-knot nematode Meloidogyne javanica. Australasian Plant Pathology 27:84-89. Ian Riley Lecturer in Nematology Department of Applied and Molecular Ecology The University of Adelaide PMB1, Glen Osmond SA 5062, Australia Phone: +61 8 8303 7259 Fax: +61 8 8379 4095 email: ian.riley@adelaide.edu.au http://www.waite.adelaide.edu.au/AME/ -----Original Message----- From: NEMA Discussion List [mailto:NEMA-L@crcvms.unl.edu] On Behalf Of cal Sent: Tuesday, 11 May 1999 18:46 To: NEMA-L@crcvms.unl.edu Subject: in vitro culturing Dear Nematologists: I am trying to culture Meloidogyne spp. in vitro on tomato roots. I succeded on culturing the tomato roots on MS medium and getting red of contamination by enough surface sterilization for the J2 but now after about one month of adding the J2 there is no any symptoms which can say that there was any infection. However, the J2 idividuals seems active on the media but starving. Tell now, there is no any galls. I will be very appreciated if somebody who have experience about that can tell me if there any conditions which i must avoid or keep to have success in getting galls. Thanks in advance. Abd El-Nasser ========================================================================= Date: Wed, 12 May 1999 10:15:20 +0300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: cal Subject: Re: in vitro culturing MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dear Dr Xiang: Thanks alot for your help. It gave me ways to rethink about several things. let us to try to find out that point you mentioned: > >Dear Dr. El-Nasser, > >There may be several reasons for your problem. > >1. The roots: Is it too old, or is it growing vigorously? Nematodes >usually prefer young growing roots. And make sure it's a susceptible >variety. The root is young tomato-root tips transferred to the media after 4-6 of culturing their seeds (castle rock tomato) on MS medium at 28 degree centigrade. >2. The medium: B5, STW, White's are great media for tomato roots, too. >Sometimes rich media such as MS can adversely affect nematode activities. You may be right about using MS media but I used it because it was mentioned in th following paper:"Hutangura P., Jones M. G. K. and Heinrich T. (1998). Optimisation of culture conditions for in vitro infection of tomato with the root-knot nematode Meloidogyne javanica. Australasian Plant Pathology 27:84-89". However, I will try to use B5 Hamborg media. >3. The nematode: Sometimes heavy sterilization of the J2 hurts the >nematodes so much that they can't behave normally. So if you doubt about >it, try some lighter procedures. Hope this helps. You should be able to >see the galling begins 2-3 days after inoculation if everything is ok. >Good luck! > >Xiang It is a good point and I doubted about that but could that affect even if I surface sterilized the eggs. I think hatching after the surface sterilization of the eggs can be considered as a good indication that the J2 is healthy and able to make infection. > Finally, I would like to thank you so much for your valuable information and hope we can keep in contact. With my best regards. Abd El-Nasser ========================================================================= Date: Wed, 12 May 1999 10:35:31 +0200 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Sergio Molinari Subject: Re: in vitro culturing In-Reply-To: <1.5.4.16.19990512101757.2387c0e8@ageri.sci.eg> MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" I didn't have particular problems infecting cultured young tomato roots with surface sterilized egg masses. I suppose that the matrix protects the eggs from toxic sterilizers. I had a huge amount of galls by this method. Sergio Molinari ========================================================================= Date: Wed, 12 May 1999 11:43:09 +0300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: cal Subject: Re: in vitro culturing MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" Content-transfer-encoding: quoted-printable Dear Sergio: Would you please let me know in details your way to that. I may find the real reasons for the troubles which I am facing. I did surface sterilization for the egg masses and the eggs hatched successfully but there was no infection. Also, did you publish any papers about that. With my best regards. Abd El-Nasser With my bets At 10:35 =D5 12/05/99 +0200, you wrote: >I didn't have particular problems infecting cultured young tomato roots >with surface sterilized egg masses. I suppose that the matrix protects >the eggs from toxic sterilizers. I had a huge amount of galls by this >method. > >Sergio Molinari > ========================================================================= Date: Mon, 17 May 1999 11:16:19 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Howard Ferris Subject: SON/ASP Registration Alert MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" This is a reminder that registration forms for the joint meeting of the American Society of Parasitologists and the Society of Nematologists are due on or before June 1, 1999 to avoid late-registration fees. If you do not have registration forms, they can be downloaded on the web from: http://biology-afs.biology.uiowa.edu/~ASP/aspcal99.pdf. Note that registration forms must be mailed, we do not have the capability for electronic registration. We look forward to seeing you in Monterey. Howard Ferris PS ignore this if you have already registered! ========================================================================= Date: Mon, 18 May 2099 09:26:38 +0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: =?hz-gb-2312?B?fntBdVBTVlJ+fQ==?= Subject: =?hz-gb-2312?B?fns7WDg0fn06IFNPTi9BU1AgUmVnaXN0cmF0aW9uIEFsZXJ0?= MIME-version: 1.0 Content-type: text/plain; charset="hz-gb-2312" Content-transfer-encoding: 7bit Hi. Dr. Liu There is a information about a meeting. Miao Zuoqing -----Original Message----- ~{7"<~HK~}: Howard Ferris ~{JU<~HK~}: NEMA-L@crcvms.unl.edu ~{HUFZ~}: 1999~{Dj~}5~{TB~}18~{HU~} 2:17 ~{VwLb~}: SON/ASP Registration Alert >This is a reminder that registration forms for the joint meeting of the >American Society of Parasitologists and the Society of Nematologists are >due on or before June 1, 1999 to avoid late-registration fees. If you do >not have registration forms, they can be downloaded on the web from: >http://biology-afs.biology.uiowa.edu/~ASP/aspcal99.pdf. >Note that registration forms must be mailed, we do not have the >capability for electronic registration. We look forward to seeing you in >Monterey. Howard Ferris PS ignore this if you have already registered! > ========================================================================= Date: Mon, 17 May 1999 20:11:49 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Xiang Huang Subject: Re: SON/ASP Registration Alert MIME-version: 1.0 Content-type: text/plain; charset="US-ASCII" Content-transfer-encoding: 7bit Dear Ferris, I am a graduate student in the Nematology Dept. at UC Riverside. I would like to apply for the student visual aid job to waive my registration fee for the SON meeting in Monterey. Thank you for your consideration! Xiang Huang UCR Nematology Riverside, CA 92521 909-787-4586 ========================================================================= Date: Sun, 30 May 1999 12:40:47 -0400 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Sarah Rosloski Subject: (Fwd) Job Posting MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Nematological job posting below. Sarah Rosloski Department of Environmental Biology University of Guelph Guelph, Ontario Canada > The following position is open at Dow AgroSciences in San Diego. Please > refer to the contact information in the notice below for any questions. > Do not respond to me as I am just the messenger! (This is the part where > we test your ability to follow instructions:) > > RESEARCH POSITIONS AVAILABLE WITH > DOW AGROSCIENCES > IN SAN DIEGO > > With over ten years of experience in cutting-edge research in plant and > microbial genetic manipulation and the recent alliance with Mycogen > Corporation, our biotechnology program is poised for substantial growth. > Dow AgroSciences' unique research effort offers that hard-to-find > balance of entrepreneurial opportunity and long-term stability. It's a > powerful combination that attracts innovative and ambitious research > professionals. > > Because we believe in the importance of moving forward to meet the needs > of a growing world population, we have made a substantial investment in > biotechnology. By researching, developing, and marketing genetically > enhanced crops, we will take a leading role in shaping the future of > global agriculture. If you would like to share in this exciting > endeavor, please consider the opportunities below. > > Nematologists /Entomologists > > B.S. and M.S. positions available with experience in Entomology or > Nematology to evaluate product concepts for plant protection. > Experience in experimental design, methods development, nematode or > insect propagation and the application of microscopy techniques to > nematodes are desirable attributes. The ideal candidate will combine > strong technical skills with superior organizational and communication > skills and apply these talents to the discovery of novel methods for > nematode and insect control. [Department Code: IAGD-5] > > > Dow AgroSciences offers an excellent salary/benefits package in an ideal > location. San Diego, California offers great year-round weather and a > wealth of cultural and recreational opportunities. Interested > candidates should send a letter of interest, resume, transcripts and > references by July 16, 1999 to: Dow AgroSciences LLC, Human Resources > Recruiting (please indicate Department Code), 9330 Zionsville Road, > Indianapolis, IN 46268-1054. Questions may also be directed to > > > Dow AgroSciences offers challenging opportunities within a stimulating > and diverse work environment. We are an affirmative action/equal > opportunity employer. Medhat Nasr, Ph.D. Research Scientist, Ontario Beekeepers' Association Dept. Environmental Biology University of Guelph Guelph, Ontario, Canada N1G 2W1 Tel: (519) 824-4120 Fax:(519)837-0442 e-mail: mnasr@evbhort.uoguelph.ca