Date: Tue, 2 Feb 1999 11:26:33 +0000 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: MARTIE BOTHA X 251 Organization: ARC - TCRI Subject: (Fwd) Changes: 14th Symposium, NSSA MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Dear colleagues - CHANGE OF VENUE AND POSTPONEMENT 14th Symposium, Nematological Society of Southern Africa, that would have been held from 8 - 12 March 1999 at Alpine Heath. This is to inform you that the venue has been cancelled, the organising committee has changed and the dates of the symposium have been postponed. The new dates (after taking as many people, and circumstances into consideration) are 30 May - 3 June 1999. The new venue will be Dikhololo, a resort some 80 km west of Pretoria. PLEASE NOTICE THAT THESE DATES ARE FINAL. We tried our utmost to accommodate as many as possible personal and other preferences. If, therefore, your programme or arrangements are upset, we humbly apologize. Please take note that you do not have to respond to this message. A circular with all relevant information on the "new" symposium will be in the mail within two weeks to all of you. You will receive with it, new registration forms, abstract particulars, deadlines, information about accommodation (cheaper, of couse), travel arrangements etc. Those of you that have already registred will not have to do so again. Neither will we require any more registration or accommodation fees from those that have already paid! Those who overpaid will be reimbursed as soon as possible. This we shall handle personally with everybody involved. If you so wish you may contact the undesigned or Alex Mc Donald at alex@igg2.agric.za or tel (+27 18 Int) 018 299 6352/369/282. Driekie Fourie driekie@igg2.agric.za My apologies, if this message is a duplicate but we just want to make sure that all interested parties are informed. Regards Martie Botha ******************************************* Dr Martie Botha Tobacco and Cotton Research Institute Private Bag X82075 0300 Rustenburg, South Africa Tel: 27-14-536 3150 Fax: 27-14-536 3113 E-Mail: MARTIE@NITK1.AGRIC.ZA ========================================================================= Date: Tue, 2 Feb 1999 11:28:50 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: gary mccallister Subject: Culturing Nematodes MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII Is there some trick to culturing free-living nematodes that we are missing. I have a student who is trying to grow Chiloplacus and Cephalobus on potatoe plug media. They either do not transfer, or die out soon after transfer. I have only worked on parasitic nematodes before and never tried this. Any suggestions? Other recipes for media? etc. ========================================================================= Date: Tue, 2 Feb 1999 14:00:58 -0500 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: David Bird Subject: Parasitic nematode meeting MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" A workshop on Parasitic Nematodes (plant and animal) will be held during the 12th International C. elegans Meeting, University of Wisconsin, Madison, June 2 - 6, 1999. Interested speakers should contact either Claude Maina or David Bird at the addresses below. No abstracts will be required for the workshop; deadline for titles will be May 1st. Information about the 12th International C. elegans Meeting can be found at: http://elegans.swmed.edu/Worm_meetings.html. We will post more information about the workshop on the meeting's web site. David Bird Plant Nematode Genetics Group Department of Plant Pathology North Carolina State University (919) 515-6813 david_bird@ncsu.edu Claude V. Maina, PhD New England Biolabs Phone - 978-927-5054 ext.251 32 Tozer Rd FAX - 978-921-1350 Beverly MA 01915-5599 e-mail - maina@neb.com ========================================================================= Date: Tue, 2 Feb 1999 15:14:01 -0500 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: David Bird Subject: Parasitic nematode meeting - adendum MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" It has just been pointed out to me (thanks Bill) that the latest C. elegans meeting Web page is at: http://www.wisc.edu/union/info/conf/celegans/celegans.html We anticipate that information about the papasite meeting will be linked to that URL soon. Best wishes, dave David McK. Bird Associate Professor Plant Nematode Genetics Group Department of Plant Pathology North Carolina State University 3415 Gardner Hall Raleigh, NC 27695-7616 Office: (919) 515-6813 Lab: (919) 515-2753 Fax: (919) 515-9500 http://www2.ncsu.edu/unity/lockers/project/plantpath/Personnel/Faculty/bir d.html ========================================================================= Date: Tue, 2 Feb 1999 22:30:10 -0500 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Ana Cunha Subject: Re: Culturing Nematodes MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" I've got a cephalobus species growing very happilly on standard c.elegans media with e.coli. Good luck, Ana > Is there some trick to culturing free-living nematodes that we are >missing. I have a student who is trying to grow Chiloplacus and >Cephalobus on potatoe plug media. They either do not transfer, or die >out soon after transfer. I have only worked on parasitic nematodes >before and never tried this. Any suggestions? Other recipes for media? >etc. ______________________________ Ana Cunha Department of Biology Imperial College, Silwood Park Ascot, Berks. SL5 7PY UK Tel: (01344) 294 335 Fax: (01344) 294 339 E-mail: a.cunha@ic.ac.uk ========================================================================= Date: Wed, 3 Feb 1999 09:00:36 +0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Michal Brzeski Organization: Instytut Warzywnictwa Subject: Re: Culturing Nematodes MIME-version: 1.0 Content-type: text/plain; charset=iso-8859-2 Content-transfer-encoding: 7bit For Chiloplacus check the following: Roy, T.K. 1973. Selection of media for culturing free living nematodes: axenic culture of Chiloplacus lentus Thorne (Nematoda: Rhabditidae). Indian Journal of Nematology, 3: 64-70. There were also some papers by Jairajpuri and his associates published either in Nematologica or Nematologia mediterranea, but I did not keep records. Peace and regards to all, Michal W. Brzeski ========================================================================= Date: Wed, 3 Feb 1999 10:22:09 +0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Paul De Ley Subject: Re: Culturing Nematodes In-Reply-To: MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" At 11:28 02/02/99 -0700, you wrote: > Is there some trick to culturing free-living nematodes that we are >missing. I have a student who is trying to grow Chiloplacus and >Cephalobus on potatoe plug media. They either do not transfer, or die >out soon after transfer. I have only worked on parasitic nematodes >before and never tried this. Any suggestions? Other recipes for media? >etc. > Hi Gary, Depending on the nematode, it is OK or not to grow them on enriched media. In the case of cephalobs, it is often unnecessary - and often counterproductive because the nemas are easily overwhelmed by any antagonistic bacteria that may co-occur with them in the soil sample. What I do myself is grow cephalobs on plain 1% water agar with 5 ug/ml cholesterol and feed with limited amounts of E. coli (strain OP50 or 9001). This definitely does not work for ALL cephalobs, but Chiloplacus and Cephalobus should be fine. For a lengthy text on culture methods for freelivers, see http://helios.bto.ed.ac.uk/mbx/fgn/worm/freecult.html Paul ========================================================================= Date: Wed, 3 Feb 1999 14:35:25 -0500 Reply-To: Sarah Rosloski Sender: NEMA Discussion List From: Sarah Rosloski Subject: Nema-Induced Druses In-Reply-To: <0021C1CF250000011B000123F70166@lanmthnt.mmm.fi> MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII I was recently examining some old papers on histopathology of resistant vs susceptible culitvars. Mc Clure et al. 1974 compared two cotton cultivars and found druses (calcium oxalate crystals) formed in both the R (Clevewilt) and S (Deltapine) roots at about 20 days, post-M. incognita infection, in the area of infection. This article appears to be the only reference to infection-induced druses in nematode-infected plant tissue that I could find. This is very interesting and I would like to discuss it with any interested plant pathologists. Calcium oxalate has been examined primarily because it is produced by pathogens and aids in subversion of the plant host. Calcium oxalates are produced by fungal pathogens, such as Sclerotinia rolfsii, and sequester calcium, acidify the cell wall and thus weaken it to aid in the ingress of the fungus. Calcium is an important signal molecule during the hypersensitive response and tying it up may also limit components of active defense. Enzymes such as the germin-like proteins have oxalate oxidase activity and are induced by plants during pathogenesis. The action of this enzyme frees calcium and produces H2O2, which is another well-known component of active defense and that also contributes to the formation of secondary cell wall, and eventuates in plant protection from oxalate secreting pathogens. The formation of druses in the infection area of Meloidogyne infected cotton may be plant-initiated or nematode initiated. The druses are found in the vicinity of the infection area of both sensitive and resistant nematode-infested plants. If it was plant induced, sequestering calcium could inhibit the muscular movement of the worm, as nematodes need calcium for muscle movement. Also, a major difference between the R and S is that the S cultivar forms secondary cell walls on the syncytia and the Resistant cultivar does not. This resistant cultivar does not show root necrosis like many other resistant plants, the nematode just dissappears. Sequestering calcium would be a limitation to the necrosis produced during the hypersensitive response. What remains to be answered in my mind is: Could the druses be initiated by the nematode, as a component of pathogenesis or even be excretory refuse? Contentions or comments concerning these ideas? Sarah Rosloski Department of Environmental Biology University of Guelph Guelph, Ontario Canada ========================================================================= Date: Thu, 4 Feb 1999 15:04:44 +0000 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: MARIETTE MARAIS Organization: NIPB RIETONDALE Subject: Request for information Comments: To: nema-l@unl.edu MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Dear colleagues Could anyone please provide us with an e-mail address or fax number for Prof Jimmy Marks of the Queen's University of Belfast. Thank you in advance Mariette Marais --------------------------------- National Collection of Nematodes Biosystematics Division ARC-Plant Protection Research Institute Private Bag X134 Pretoria 0001 South Africa Tel: +27 12 3293269 Fax: +27 12 3293278 Rietmm@plant2.agric.za ========================================================================= Date: Wed, 10 Feb 1999 17:00:40 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: "Michael A. McClure" Subject: Third English Language International Nematology Symposium MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dear Friends and Colleagues: On behalf of the Russian Society of Nematologists, I would like to invite you to participate in the Third English Language International Nematology Symposium. It will take place from August 21-27, 1999, at the Zoological Institute of the Russian Academy of Sciences, situated in the historic city center of beautiful St. Petersburg. The scientific program will include the following topics: Molecular Biology & Genetics Phylogeny & Systematics Control and Suppressants Identification and Diagnosis Ecology and Host-Parasite Relationships Biogeography & Biodiversity Methods & Techniques Computers & Teaching A symposium-wide, all-day tour of the famed Peterhoff Palace-Museum-Reserve will highlight the social activities and a complete social and cultural program is planned for accompanying persons. Please examine the Meeting Announcement and registration materials that can be found at: http://ianrwww.unl.edu/ianr/plntpath/nematode/son/sonhome.htm Registration fees are all-inclusive: symposium, hotel, meals, excursions, social events, airport transfers, etc. Preregistration and abstract deadline is April 15, 1999. If you don't have a WWW connection or cannot download either of the Symposium files (WordPerfect and MS Word), let me know and I will send you a paper copy. My wife and I attended the Symposium in 1997 and were simply thrilled with the opportunity to visit this fabulously interesting country and meet the Russian nematologists, previously known to us only from their literature. It was an unforgettable experience and an unparalleled chance to learn what is going-on today in Russian nematology as well as to see first-hand the many changes that are taking place within the country. We will be there again this year and look forward the pleasure of your company. A guided, post-symposium tour of the "Golden Ring" also is planned. Participants will travel by train and coach to some of the historically and culturally rich towns of this area. The size of the Golden Ring tour group is limited, so make your reservations early (before April 15, 1999). For questions or additional information, call, write, or FAX: M. A. McClure Department of Plant Pathology 204 Forbes Bldg. University of Arizona Tucson, AZ 85721 USA Tel/FAX: +520-621-7161 ========================================================================= Date: Thu, 11 Feb 1999 21:09:12 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: John Webster Subject: Re: Third English Language International Nematology Symposium In-Reply-To: <3.0.32.19990210170040.007e5e90@ag.arizona.edu> MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Hi,Mike! Thanks for all the info on the Russian meeting. However, although I was able to retrieve material off your earlier message to me I have not been able to access your latest attachments. Could please FAX me or mail me the full info. Thanks My FAX, if you want it, is 604.291.3496 Lyn and I will probably go on the tour afterwards. Will let you know. Cheers, John. At 05:00 PM 2/10/99 -0700, you wrote: >Dear Friends and Colleagues: > >On behalf of the Russian Society of Nematologists, I would like to invite >you to participate in the Third English Language International Nematology >Symposium. It will take place from August 21-27, 1999, at the Zoological >Institute of the Russian Academy of Sciences, situated in the historic >city center of beautiful St. Petersburg. > >The scientific program will include the following topics: > >Molecular Biology & Genetics Phylogeny & Systematics >Control and Suppressants Identification and >Diagnosis Ecology and Host-Parasite Relationships Biogeography & >Biodiversity Methods & Techniques Computers & >Teaching > >A symposium-wide, all-day tour of the famed Peterhoff >Palace-Museum-Reserve will highlight the social activities and a complete >social and cultural program is planned for accompanying persons. > >Please examine the Meeting Announcement and registration materials that >can be found at: > >http://ianrwww.unl.edu/ianr/plntpath/nematode/son/sonhome.htm > >Registration fees are all-inclusive: symposium, hotel, meals, >excursions, social events, airport transfers, etc. Preregistration and >abstract deadline is April 15, 1999. > >If you don't have a WWW connection or cannot download either of the >Symposium files (WordPerfect and MS Word), let me know and I will send >you a paper copy. > >My wife and I attended the Symposium in 1997 and were simply thrilled >with the opportunity to visit this fabulously interesting country and >meet the Russian nematologists, previously known to us only from their >literature. It was an unforgettable experience and an unparalleled chance >to learn what is going-on today in Russian nematology as well as to see >first-hand the many changes that are taking place within the country. We >will be there again this year and look forward the pleasure of your >company. > >A guided, post-symposium tour of the "Golden Ring" also is planned. >Participants will travel by train and coach to some of the historically >and culturally rich towns of this area. The size of the Golden Ring tour >group is limited, so make your reservations early (before April 15, >1999). > >For questions or additional information, call, write, or FAX: > >M. A. McClure >Department of Plant Pathology >204 Forbes Bldg. >University of Arizona >Tucson, AZ 85721 >USA >Tel/FAX: +520-621-7161 > ========================================================================= Date: Fri, 12 Feb 1999 09:29:24 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: gary mccallister Subject: Entomopathogenic Nematodes MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII I have a student who has been trying to isolate entomopathogenic nematodes from our high desert soils, without success. Maybe it is the cold and the time of year out here in Colorado. He has plans to continue trying but is getting discouraged. Also, he would like to learn how to culture them if and when we can find some. I have never worked with these critters myself (only animal parasitic worms and Thelastomids in cockroaches) and so think perhaps we should get some worms and learn more about culturing them while we extend our search into the summer months. Does anyone know of a source where we could purchase or beg some Steinernema or Hetterorhabditis culture for starters? ========================================================================= Date: Fri, 12 Feb 1999 08:45:27 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Harry Kaya Subject: Re: Entomopathogenic Nematodes MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Gary: We have the standard steinernematids and heterorhabditids and can send you starter cultures. Send me your mailing address and it will go out on Tuesday as Monday is a holiday. harry At 09:29 AM 2/12/99 -0700, you wrote: > I have a student who has been trying to isolate entomopathogenic >nematodes from our high desert soils, without success. Maybe it is the >cold and the time of year out here in Colorado. He has plans to continue >trying but is getting discouraged. Also, he would like to learn how to >culture them if and when we can find some. I have never worked with >these critters myself (only animal parasitic worms and Thelastomids in >cockroaches) and so think perhaps we should get some worms and learn more >about culturing them while we extend our search into the summer months. >Does anyone know of a source where we could purchase or beg some >Steinernema or Hetterorhabditis culture for starters? > > Harry K. Kaya Department of Nematology University of California, Davis Davis, CA 95616-8668 E-Mail: hkkaya@ucdavis.edu Tel: (530) 752-1051 Fax: (530) 752-5809 ========================================================================= Date: Mon, 15 Feb 1999 10:20:20 +1100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Robin Bedding Subject: Re: Entomopathogenic Nematodes MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" May I suggest that he collects soil samples from some more hospitable environments to convince himself that the method (presumably galleria baiting) works. Also I trust that he is moistening the desert soil prior to baiting. Good luck Robin Bedding At 09:29 12/02/99 -0700, you wrote: > I have a student who has been trying to isolate entomopathogenic >nematodes from our high desert soils, without success. Maybe it is the >cold and the time of year out here in Colorado. He has plans to continue >trying but is getting discouraged. Also, he would like to learn how to >culture them if and when we can find some. I have never worked with >these critters myself (only animal parasitic worms and Thelastomids in >cockroaches) and so think perhaps we should get some worms and learn more >about culturing them while we extend our search into the summer months. >Does anyone know of a source where we could purchase or beg some >Steinernema or Hetterorhabditis culture for starters? > > ========================================================================= Date: Wed, 17 Feb 1999 11:53:34 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Howard Ferris Subject: ASP/SON 1999 - Reminder and Information MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" The joint meeting of the American Society of Parasitologists and the Society of Nematologists will be held in Monterey, California on July 6-10, 1999. The Call for Papers, Meeting Announcement and relevant forms for registration are now available on the Web at: http://biology-afs.biology.uiowa.edu/~ASP/aspcal99.pdf Further information can be obtained from: http://ucdnema.ucdavis.edu/imagemap/nemmap/sonasp.htm Abstracts for papers are due March 1, 1999. They can be submitted by mail, diskette, or electronically at: http://www-museum.unl.edu/asp/ We look forward to seeing you in Monterey. Howard Ferris ========================================================================= Date: Tue, 23 Feb 1999 18:10:56 +0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Marion Link MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Does anybody have experiences in handling parasitic nematodes (Strongylida) which were collected 30 years ago? I have the following questions: Is there any disadvantage in transferring specimens from formalin (in which they were stored for 30 years) into ethanol 70-80% with 1% glycerine? Would you know of any method to soften specimens so that they can be manipulated more easily for microscopic slides? ---------------------------------------- Marion Link Museum fuer Naturkunde Institut fuer Systematische Zoologie Invalidenstr. 43; 10115 Berlin; Germany Tel. +49/30/20938525 Fax. +49/30/20938528 ========================================================================= Date: Fri, 26 Feb 1999 15:30:55 +0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Michal Brzeski Organization: Instytut Warzywnictwa Subject: book announcement MIME-version: 1.0 Content-type: text/plain; charset=iso-8859-2 Content-transfer-encoding: 7bit Book announcement The new book has been published: M. W. Brzeski - Nematodes of Tylenchina in Poland and temperate Europe. Publisher: Muzeum i Instytut Zoologii PAN, Warszawa, 397 pp., ISBN 83-85192-84-0 The book contains descriptions, illustrations and keys for 50 genera and 305 species found in the region. Five new species are described and some new synonyms are proposed. Beside, there are check lists and tables with some diagnostic features of species of the genera Filenchus, Malenchus, Coslenchus, Ditylenchus, Geocenamus, Tylenchorhynchus, Rotylenchus and Paratylenchus of world fauna. The price of the book is US$ 70 for organizations, institutions and libraries, or US$ 50 for individuals (sent to home address), or US$ 28 for students (statement from the university professor required to prove student's status). The price includes packing and shipping by regular mail. For air mail delivery in Europe add US$ 8, outside Europe add US$ 13. Cheques should be made payable for: Muzeum i Instytut Zoologii PAN, Wilcza 64, 00-679 Warszawa, Poland, and orders should be sent to this address with indication 'Biblioteka'. Michal W. Brzeski ========================================================================= Date: Sat, 27 Feb 1999 01:22:12 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Nethi Somasekhar Subject: mounting of EPN MIME-version: 1.0 Content-type: text/plain I would like to prepare permanent mounts of Heterorahbditid and Steinernematid nematode isolates for identification. Can any one tell me what is the best method for killing, fixing,processing to glycerol and mounting of these nematodes. N. Somasekhar ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com ========================================================================= Date: Sat, 27 Feb 1999 01:26:14 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Nethi Somasekhar Subject: screening for lesion nematode resistance MIME-version: 1.0 Content-type: text/plain I am screening sugarcane clones for resistamce to lesion nematode, Pratylenchus zeae. I would like to know how to rate these clones as susceptible and resistance to lesion nematode. Is there any standard scale like root-knot index in case of root-knot nematode. In sugarcane it is difficult to count root-lesions as roots become thick and wiry after maturity. Can any one tell me about the right method for rating sugarcane clones for resistance/susceptible to lesion nematode N. Somasekhar ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com ========================================================================= Date: Sun, 28 Feb 1999 20:31:11 +0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Paul Speijer Subject: Re: screening for lesion nematode resistance In-Reply-To: <19990227092616.18804.qmail@hotmail.com> MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dear Somasekhar, You could use the same method I use for scoring root necrosis on banana roots (caused by Pratylenchus spp., R. similis and H. multicinctus). INIBAP has produced a manual with clear picktures: Speijer, P.R. and Waele, D. De (1997).Screening of Musa germplasm for resistance and tolerance to nematodes, INIBAP Technical Guidelines 1, International Network for the Improvement of Banana and Plantain, Montpellier, France, 47p. All the best, Paul At 01:26 AM 2/27/99 -0800, you wrote: >I am screening sugarcane clones for resistamce to lesion nematode, >Pratylenchus zeae. I would like to know how to rate these clones as >susceptible and resistance to lesion nematode. Is there any standard >scale like root-knot index in case of root-knot nematode. In sugarcane it >is difficult to count root-lesions as roots become thick and wiry after >maturity. Can any one tell me about the right method for rating sugarcane >clones for resistance/susceptible to lesion nematode > >N. Somasekhar > >______________________________________________________ >Get Your Private, Free Email at http://www.hotmail.com > >