log0009

Date: Mon, 11 Sep 2000 12:24:07 +1000
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
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From: Garry Rosewarne <g.rosewarne@PI.CSIRO.AU>
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I want to infect Linum usitatissimum (flax or linseed) with a
Meloidogyne
sp. Which species should I use? I have cultures of M. javanica, M. hapla
and M. trifoliophila.

Thanks
Garry

Dr. Garry Rosewarne
Plant Industry
CSIRO, Black Mountain

GPO Box 1600
Canberra, ACT 2601

Phone: (02) 6246 4947
Fax: (02) 6246 5000
email: garry.rosewarne@pi.csiro.au
=========================================================================
Date: Mon, 11 Sep 2000 08:54:37 -0700
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: Howard Ferris <hferris@UCDAVIS.EDU>
In-Reply-To: <200009110126.MAA14280@pican.pi.csiro.au>
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flax is listed as a host of M. javanica and M. hapla; I'd be inclined to
use M. javanica, but it depends on your objective.
Howard

At 12:24 PM 09/11/2000 +1000, you wrote:
>I want to infect Linum usitatissimum (flax or linseed) with a Meloidogyne
>sp. Which species should I use? I have cultures of M. javanica, M. hapla
>and M. trifoliophila.
>
>Thanks
>Garry
>
>
>Dr. Garry Rosewarne
>Plant Industry
>CSIRO, Black Mountain
>
>GPO Box 1600
>Canberra, ACT 2601
>
>Phone: (02) 6246 4947
>Fax: (02) 6246 5000
>email: garry.rosewarne@pi.csiro.au
=========================================================================
Date: Thu, 14 Sep 2000 09:53:54 +0200
Reply-To: Tom.Bongers@Medew.NEMA.WAU.NL
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: "to Tom.Bongers@medew.nema.wag-nur.nl"
<Tom.Bongers@MEDEW.NEMA.WAU.NL>
Subject: MI-site update
Comments: To: NEMA-L@unl.edu
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Site www.spg.wau.nl/nema/MI_lit.htm (overview of Nematode Maturity Index
literature) has just been updated.

Tom
=========================================================================
Date: Wed, 20 Sep 2000 14:54:04 +1200
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: David Wharton <david.wharton@STONEBOW.OTAGO.AC.NZ>
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We have been awarded funding for a postdoctoral fellow (3 years) to
work on ice active proteins in an Antarctic nematode that survives
intracellular freezing. The project will involve the isolation and
sequencing of a protein which is involved in the ability of an
Antarctic nematode to survive intracellular freezing and the
investigation of its properties and evolution. The appointee will
have experience in protein purification and molecular biology
techniques. I'd welcome any preliminary expressions of interest in
the position and will send you the official advert (and post it to
the list) when it's available.

David Wharton, Department of Zoology, University of Otago, Dunedin,
New Zealand (david.wharton@stonebow.otago.ac.nz)

I will be away at a conference until Oct 2nd, prior to then please
reply to my coinvestigator, Dr. Craig Marshall, Department of
Biochemistry (craig.marshall@stonebow.otago.ac.nz)

--
____________________
David Wharton
Department of Zoology
University of Otago
P.O. Box 56
Dunedin, New Zealand.
Tel (064) (03) 479 7963
Fax (064) (03) 479 7584
david.wharton@stonebow.otago.ac.nz
http://www.otago.ac.nz/Zoology/Index.html
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<div>We have been awarded
funding for a postdoctoral fellow (3 years) to work on ice active
proteins in an Antarctic nematode that survives intracellular
freezing. The project will involve the isolation and sequencing of a
protein which is involved in the ability of an Antarctic nematode to
survive intracellular freezing and the investigation of its
properties and evolution.  The appointee will have experience in
protein purification and molecular biology techniques. I'd welcome
any preliminary expressions of interest in the position and will send
you the official advert (and post it to the list) when it's
available. 
 
David Wharton, Department of Zoology, University of Otago, Dunedin,
New Zealand (david.wharton@stonebow.otago.ac.nz) 
 
I will be away at a conference until Oct 2nd, prior to then please
reply to my coinvestigator, Dr. Craig Marshall, Department of
Biochemistry (craig.marshall@stonebow.otago.ac.nz) 
</div>

<div>-- 
____________________ 
David Wharton 
Department of Zoology 
University of Otago 
P.O. Box 56 
Dunedin, New Zealand. 
Tel (064) (03) 479 7963 
Fax (064) (03) 479 7584 
david.wharton@stonebow.otago.ac.nz 
http://www.otago.ac.nz/Zoology/Index.html</div>
</body>
</html>
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Date: Mon, 25 Sep 2000 11:06:22 +0000
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: David Trudgill <dtrudg@SCRI.SARI.AC.UK>
Subject: Bursaphelenchus xylophilus damage - can anyone lend a
slide?

<color><param>0100,0100,0100</param>Dear Colleagues,

I am seeking a slide to copy showing a panoramic view of pine
trees killed by <italic>B</italic>. <italic>xylophilus. </italic>If
anyone can help, I would be gratefu
and will return the slide immediately - that is a promise!

Many thanks David Trudgill<italic> </italic>

<nofill>
=========================================================================
Date: Mon, 25 Sep 2000 12:12:08 +0200
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: "Zullini Aldo - Dip. di Biotecnologie e Bioscienze"
<aldo.zullini@UNIMIB.IT>
Subject: Re: Bursaphelenchus xylophilus damage - can anyone lend a
slide?
In-Reply-To: <314120E1402@law.scri.sari.ac.uk>
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Dear Dr Trudgill,

Maybe Dr. Laura Ambrogioni (Firenze) has such slide.

Aldo Zullini

Aldo Zullini, Universita' Milano-Bicocca, Dip.Biol., P.zza d.Scienza 2,
20126 MILANO(Greco). tel.0039 02 6448 3429; fax 0039 02 6448 3565
=========================================================================
Date: Tue, 26 Sep 2000 12:11:51 +0100
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: Mark Blaxter <mark.blaxter@ED.AC.UK>
Subject: Three positions in the Blaxter Nematode Genomics/Genetics
lab in
Edinburgh
Comments: To: nema-l@unl.edu
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Three positions in the Blaxter Nematode Genomics/Genetics lab in
Edinburgh

Three positions are available in Edinburgh to join Mark Blaxter's
Nematode Genetics/Genomics group. See
http://www.ed.ac.uk/~mbx/blaxlab.html for an overview of what we do.

The posts are as follows:

Wellcome Trust funded Postdoctoral position on the Nematode EST program.

2 years, PDRA 1A scale (starting salary up to =A322,579). Available
immediat=
ely.

We are performing EST sequencing on a set of five parasitic nematode
species as part of an international project to identify new drug
targets and vaccine candidates in these economically and socially
important species. cDNA libraries are constructed from all lifecycle
stages of the parasite, and, where possible, from dissected tissues.
The EST sequencing is carried out in collaboration with the Sanger
Centre, Cambridge. We also perform bioinformatics analyses of the
sequences, comparing the datasets to the fully sequenced genome of
Caenorhabditis elegans and each other. The genomics section of the
Blaxter lab is currently 4 people, including a dedicated
bioinformatician and both genomic and cDNA sequencing/analysis. The
post would suit a postdoc with skills in cDNA library construction
who wanted to take a leading role in using model organism data for
understanding human and animal health issues and in exploiting the C.
elegans genome project.

Wellcome Trust funded Postdoctoral position: Sequencing the genome of
the Wolbachia endosymbiont of Onchocerca volvulus

1 year, PDRA 1A scale (starting salary from =A318,731). Available
immediatel=
y.

The human infective filarial nematodes that cause elephantiasis and
river blindness are still common pathogens in developign countries
(120 million cases currently). We are actively engaged in using
genomics methods to discover new drug targets and vaccine candidates
for these diseases. One exciting recent development is the
rediscovery of the endosymbiotic bacteria of filarial nematodes.
These bacteria are closely related to the Wolbachia endosymbionts of
insects. We have been funded as coordinators of an international
effort to sequence the 1 Mb genome of the endosymbiont bacterium of
the human parasite Onchocerca volvulus. The post offered will involve
construction and verification of large insert genomic libraries from
Onchocerca and the isolation of Wolbachia derived genomic clones from
these by hybridisation. A physical map of the genome will be
constructed and submitted for sequencing by the Pathogens Sequencing
Unit at the Sanger Centre, Cambridge. The successful applicant will
join our growing genomics group in Edinburgh, and will have
opportunities for participation in all the activities of the group.
In particular we are collaborators in the NEB/NIH funded consortium
sequencing the endosymbiont of another filarial nematode, Brugia
malayi, and we would expect significant synergy between these two
projects.

NERC funded Postdoctoral Position: Development of a Molecular Barcode
System for the Identification of Nematodes

18 months, PDRA 1A scale (starting salary from =A318,731). Available
Immediateley.

Nematodes are abundant and speciose members of the meiofauna, and
must play some (significant) role in ecosystem processes. However,
due to their small size and morphologica;l conservatism, they are
little studied for this. We are part of a major NERC initiative (see
http://mwnta.nmw.ac.uk/soilbio/index.html) examining the role of
biodiversity in ecosystem processes on a Scottish uplands study site.
We are developing a "molecular barcode" system for nematode
identification, and biodiversity assessment, using the small subunit
ribosomal RNA gene (see http://www.ed.ac.uk/~mbx/syrup/syrup.html for
more information). We have an opening for a postdoctoral worker on
this project. The successful applicant's role will be to build a
database of Scottish soil nematode "molecular barcodes" and relate
this to known taxa (species) defined morphologically. (We have
already identified at least one new species.) The next step will be
to devise hybridisation and PCR-screening based methods for examining
the changes in diversity and abundance following perturbations of the
soil system, and integtrating these findings with the other NERC
projects on the same site (examining other soil fauna and flora, and
nutrient flow).

Please contact Mark Blaxter at mark.blaxter@ed.ac.uk (ph 0131 650
5450 fax 0131 650 7489) if you are interested. Please pass this
message on to anyone you think might be interested.
--
_________________________________________________
Dr. Mark Blaxter email Mark.Blaxter@ed.ac.uk
Institute of Cell, Animal and Population Biology
Ashworth laboratories, Room 311
King's Buildings, University of Edinburgh,
West Mains Road, EDINBURGH EH9 3JT, UK
phone: (+44) 131 650 6760 **NEW** Fax :...650 7489
see http://www.ed.ac.uk/~mbx/blaxlab.html
or http://www.nematodes.org

~ may all beings be happy ~
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<html><head><style type=3D"text/css"></style><title>Three positions in the Blaxter Nematode
Genomics/Genet</title></head><body>
<div>Three positions
in
the Blaxter Nematode Genomics/Genetics lab in Edinburgh 
 
Three positions are available in Edinburgh to join Mark Blaxter's
Nematode Genetics/Genomics group. See
http://www.ed.ac.uk/~mbx/blaxlab.html for an overview of what we
do. 
 
The posts are
as
follows: 
 
Wellcome
Trust
funded Postdoctoral position on the Nematode EST program. 
 
2 years,
PDRA
1A scale (starting salary up to =A322,579). Available immediately. 
 
We are performing EST sequencing on a set of five parasitic nematode
species as part of an international project to identify new drug
targets and vaccine candidates in these economically and socially
important species. cDNA libraries are constructed from all lifecycle
stages of the parasite, and, where possible, from dissected tissues.
The EST sequencing is carried out in collaboration with the Sanger
Centre, Cambridge. We also perform bioinformatics analyses of the
sequences, comparing the datasets to the fully sequenced genome of
Caenorhabditis elegans and each other. The genomics section of the
Blaxter lab is currently 4 people, including a dedicated
bioinformatician and both genomic and cDNA sequencing/analysis. The
post would suit a postdoc with skills in cDNA library construction who
wanted to take a leading role in using model organism data for
understanding human and animal health issues and in exploiting the
C. elegans genome project. 
 
Wellcome
Trust
funded Postdoctoral position: Sequencing the genome of the
Wolbachia endosymbiont of Onchocerca volvulus 
 
1
year,
PDRA 1A scale (starting salary from =A318,731). Available
immediately. 
 
The human infective filarial nematodes that cause elephantiasis and
river blindness are still common pathogens in developign countries
(120 million cases currently). We are actively engaged in using
genomics methods to discover new drug targets and vaccine candidates
for these diseases. One exciting recent development is the rediscovery
of the endosymbiotic bacteria of filarial nematodes. These bacteria
are closely related to the Wolbachia endosymbionts of insects.
We have been funded as coordinators of an international effort to
sequence the 1 Mb genome of the endosymbiont bacterium of the human
parasite Onchocerca volvulus. The post offered will involve
construction and verification of large insert genomic libraries
from Onchocerca and the isolation of Wolbachia derived
genomic clones from these by hybridisation. A physical map of the
genome will be constructed and submitted for sequencing by the
Pathogens Sequencing Unit at the Sanger Centre, Cambridge. The
successful applicant will join our growing genomics group in
Edinburgh, and will have opportunities for participation in all the
activities of the group. In particular we are collaborators in the
NEB/NIH funded consortium sequencing the endosymbiont of another
filarial nematode, Brugia malayi, and we would expect
significant synergy between these two projects. 
 
NERC funded
Postdoctoral Position: Development of a Molecular Barcode System for
the Identification of Nematodes 
 
18 months,
PDRA 1A scale (starting salary from =A318,731). Available
Immediateley. 
 
Nematodes are abundant and speciose members of the meiofauna, and must
play some (significant) role in ecosystem processes. However, due to
their small size and morphologica;l conservatism, they are little
studied for this. We are part of a major NERC initiative (see
http://mwnta.nmw.ac.uk/soilbio/index.html) examining the role of
biodiversity in ecosystem processes on a Scottish uplands study site.
We are developing a "molecular barcode" system for nematode
identification, and biodiversity assessment, using the small subunit
ribosomal RNA gene (see http://www.ed.ac.uk/~mbx/syrup/syrup.html for
more information). We have an opening for a postdoctoral worker on
this project. The successful applicant's role will be to build a
database of Scottish soil nematode "molecular barcodes" and
relate this to known taxa (species) defined morphologically. (We have
already identified at least one new species.) The next step will be to
devise hybridisation and PCR-screening based methods for examining the
changes in diversity and abundance following perturbations of the soil
system, and integtrating these findings with the other NERC projects
on the same site (examining other soil fauna and flora, and nutrient
flow).</div>
<div> 
Please contact
Mark Blaxter at mark.blaxter@ed.ac.uk (ph 0131 650 5450 fax 0131 650
7489) if you are interested. Please pass this message on to anyone you
think might be interested.</div>

<div>-- 
_________________________________________________ 
Dr. Mark Blaxter   email  Mark.Blaxter@ed.ac.uk 
Institute of Cell, Animal and Population Biology 
Ashworth laboratories, Room 311 
King's Buildings, University of Edinburgh, 
West Mains Road, EDINBURGH  EH9 3JT, UK 
phone: (+44) 131 650 6760  **NEW** Fax :...650 7489 
see  http://www.ed.ac.uk/~mbx/blaxlab.html 
or   http://www.nematodes.org 
 
         ~ may all beings be
happy ~</div>
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Date: Wed, 27 Sep 2000 16:13:15 +0930
Reply-To: ian.riley@adelaide.edu.au
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: "Ian T. Riley" <ian.riley@ADELAIDE.EDU.AU>
Subject: Nematology training opportunity
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An opportunity exists through the Crawford Fund for a scientist or
technician from a developing country to undertake short term nematology
training (up to 3 months) in Adelaide, South Australia.

The Crawford Fund was established in 1989 by The Australian Academy of
Technological Sciences and Engineering to increase the number of trained
technicians, technologists and scientists who can undertake useful and
effective R&D activities to improve food production and processing in
developing countries. In May this year the Crawford Fund of South
Australia
was established with grants from State and Federal governments and is
seeking projects to support.

The fund offers training awards which cover return airfares and
accommodation for up to three months in Australia and are designed to
train
scientists and technicians from developing countries in specific
techniques
that they can then apply in their home countries to increase food
production.

If you feel that a period of training in nematology would meet the
Crawford
Fund objectives and you would like to discuss the possibility, please
provide brief biographical details, outline training needs and details
of
potential benefits.

If it appears that the combined nematology groups at the Waite Campus,
Adelaide can meet the training needs and a meritorious case can be made
to
the Crawford Fund, I would be pleased to facilitate such an application.

Dr Ian Riley
Lecturer in Nematology
Department of Applied and Molecular Ecology
The University of Adelaide
PMB1, Glen Osmond SA 5062, Australia
Phone: +61 8 8303 7259
Fax: +61 8 8379 4095
email: ian.riley@adelaide.edu.au
http://www.waite.adelaide.edu.au/AME/
=========================================================================
Date: Wed, 27 Sep 2000 09:05:33 -0700
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: Ron Ykema <RONY@SAL.AH.STATE.AZ.US>
Subject: Ozone for nematode eradication in waters; other
alternatives
MIME-version: 1.0
Content-type: text/plain; charset="iso-8859-1"

Colleagues:

I would appreciate any ideas or suggestions for the following:

Situation: A greenhouse-like facility has been sampled and found to
contain
Rotylenchulus reniformis and Radopholus similis. The water used for
irrigation on the plants is recycled through a sealed reservoir
(>200,000
gal). Over time, the total dissolved salt concentrations in this water
has
been steadily rising, and the 'owner' wishes to flush, and then
replenish
the reservoir with new water. The flushed water is intended to be used
on
the landscaping surrounding the facility.

What are some efficacious methods of treatment of this flush water to
ensure
the elimination of these nematodes and eggs prior to distribution of the
water to the landscape plantings outside this facility?

Can the use of ozone be effective? If so, what concentration and
duration
of exposure? On a much smaller scale, solar heating of small volumes of
water in a constricted tube proved effective.

Any alternative treatments? References would be greatly appreciated.

Thanking you in advance for your assistance.

Ron Ykema
Plant Pathologist
Arizona Department of Agriculture
State Agricultural Laboratory
2422 West Holly
Phoenix, AZ 85009

Tel: 602-253-1920
Fax: 602-253-2247
=========================================================================
Date: Wed, 27 Sep 2000 13:01:50 -0400
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: Leah Millar <Leah.C.Millar@USDA.GOV>
Subject: NAPPO Alert System
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Boundary="0__=ZO5ySrTLSB18ORUEtnprO3IMg5MiVX9L816dVm3UA4pWX4Wad6VGMjdP"

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The North American Plant Protection Organization (NAPPO) would like to
request
your help in determining appropriate plant parasitic nematode pests for
posting
on its new Phytosanitary Alert System, which is to go public by November
2000.
This alert system is a website that will post alerts on emerging pest
threats to
North America, enabling more effective exclusion and detection of these
pests.
This site was designed to provide timely pest alerts to interested
exclusion
authorities, including port of entry inspectors. This site may also be
of
interest to university and industry researchers. The main criteria for
pests
posted on this site are: 1) that the pest not be present or widely
distributed
in the U.S., Canada, and/or Mexico, and that 2) the pest has had
increasing
potential of being introduced into one or more of these countries. Signs
that a
pest
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?s potential for introduction has increased may include, among others: =
the
geographic distribution of the pest has changed, there is an increase i=
n
interceptions of the pest at ports of entry, or the pest has appeared o=
n a
previously unaffected commodity. NAPPO will be sure to credit any info=
rmation
utilized on the website. Please submit any suggestions/information/que=
stions
to: Leah Millar (email: leah.c.millar@usda.gov phone: 919-513-2495, fax=
:
919-513-1995). Thank you for your assistance.
=

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Date: Wed, 27 Sep 2000 13:44:28 -0700
Reply-To: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: Jay Burr <burr@SFU.CA>
Subject: Re: Three positions in the Blaxter Nematode
Genomics/Genetics lab
in
In-Reply-To: <a0500190bb5f634e8e300@[129.215.191.36]>
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Hi Mark,

I was very interested to read about these interesting projects. WRT the
molecular barcode project, would "soil nematodes" include Mermis
nigrescens? I thought it might be interesting to conpare Scottish and N
American varieties. Cobb gave them different species names. I think
they are regarded as the same species now, but there is no real evidence
for this. The vanfleteren lab has the SS rRNA sequence of the n amer
variety.

Best regards,

- Jay -
=========================================================================
Date: Thu, 28 Sep 2000 10:16:28 +0200
Reply-To: Tom.Bongers@Medew.NEMA.WAU.NL
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: "to Tom.Bongers@medew.nema.wag-nur.nl"
<Tom.Bongers@MEDEW.NEMA.WAU.NL>
Subject: ...no subject...
MIME-version: 1.0
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In 1987 Gregor Yeates gave a series of lectures at the Wageningen
University,
which strongly stimulated soil ecological work. We still have a number
of
readers available, titled: Resource utilization by Nematodes, notes for
a
series of lectures given in the Nematology Department. If you are
interested,
they are free, please contact Anette.Angelino@Medew.Nema.WAU.nl
=========================================================================
Date: Thu, 28 Sep 2000 11:13:57 +0200
Reply-To: Tom.Bongers@Medew.NEMA.WAU.NL
Sender: NEMA Discussion List <NEMA-L@CRCVMS.UNL.EDU>
From: "to Tom.Bongers@medew.nema.wag-nur.nl"
<Tom.Bongers@MEDEW.NEMA.WAU.NL>
Subject: Annette instead of Anette
Comments: cc: aldo.zullini@unimib.it
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I'm sorry, Annette lost an 'N'. For a copy of Gregors reader contact
Annette.Angelino@medew.nema.wau.nl