Date sent: Wed, 09 Feb 2000 15:48:46 -0600 From: "L-Soft list server at UNIVERSITY OF NE - LINCOLN (1.8d)" Subject: File: "NEMA-L LOG0001" To: Allen Szalanski ========================================================================= Date: Tue, 4 Jan 2000 22:23:33 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Kaiji Hu Subject: Research Questions (fwd) MIME-version: 1.0 Content-type: text/plain; charset=iso-8859-1 Content-transfer-encoding: 8bit DD Dear colleagues: Could you help to answer some of the following questions about entomopathogenic nematodes? Thank you for your attention. K. Hu Dept. Biological Sciences Simon Fraser University Burnaby, BC, V5A 1S6 Canada Forwarded message: > From jdsm@televar.com Wed Dec 29 12:22:13 1999 > Message-ID: <386A6BB6.5284@televar.com> > Date: Wed, 29 Dec 1999 12:14:48 -0800 > From: Jack Massie > Reply-To: jdsm@televar.com > X-Mailer: Mozilla 3.0-C-AICK1-2 (Macintosh; U; PPC) > MIME-Version: 1.0 > To: Kaiji@sfu.ca > Subject: Research Questions > Content-Type: text/plain; charset=iso-8859-1 > Content-Transfer-Encoding: 8bit > > Dr. Hu, > > My name is Jack S. Massie and I am the Research Science teacher at > Omak > High School in Washington State (Omak, WA). I have 21 students doing > research and one of them is involved in a project dealing with thermal > hysteresis and AFP's in nematodes/insects. This student is Boyd Massie. > My job is to advise my students on procedures and analysis > methods > for their project experimentation--I find myself needing answers to > questions so I can help them. Right now Boyd's questions are in need of > some specific attention. Would you be willing to read and consider > these questions? > At Omak, we are a small school and are 3 hours from a major > university > BUT I do have access to some equipment loan programs that we have used > in the past from Washington State University's Eisenhower Loan Program. > Plus, we have been successful in the past in statewide science > competitions, so students are very positive about what they can > accomplish. > Here are my questions : > > 1) What is the specific method to measure proteins in connection with > AFP's? Are there indirect methods of comparison one could use? > > 2) What is the definition of nucleators/nucleation sites as it pertains > to this project? > > 3) A nanolitre osmometer was suggested by one professor--are these > rare? Is there another method to measure thermal hysteresis? > > 4) Inoculative freezing needs clarification to me. Would glycerin or a > carbohydrate help prevent inoculative freezing and what is supercooled > water compared to a supercooled animal? > > 5) Boyd worked with entomopathogenic nematodes last year > (Heterorhabditis and Steinernema) and after reading about some success > in storing EPN's in liquid nitrogen and their natural cold tolerance, > would experimenting with varying amounts of glycerol or sugars in > solution with EPN's have any effect on their survival rate? Is this a > direction that will dead end or does it have some possibilities? These > EPN's could be reared on hosts that were in several variables?? Can > tolerance be built up/ acclimation? Does it matter what the host is > fed? In what temps or humidity reared? > > 6) If one could induce cryoprotective production, could (it) be measured > or could the tolerant strains be isolated--would a project of 3 months > have time to isolate more protected individuals? > > 7) Infective larvae more or less cold tolerant depending if reared in > Galleria or soya or other liquid media--related to nutritional status?? > > 8) ??? Indirect method--EPN's fed on hosts vs. those in other > media--would they show protein pattern differences if gel > electrophoresis was used? Proteins found in one but not in the other > could point to EPN's getting AFP's from insects like Galleria or > Tenebrio? > > 9) Get extract from freeze-tolerant insect and rear EPN's in that > solution?? > > 10) Another idea was--would a yeast, mold, bacteria ?? that was > experi-mentally subjected to increasingly colder temps. show some > individuals that survived and those would be more "tolerant" than the > majority that died and . . . > > As you can read, I have many questions. My main concern is that I help > my student to move in a productive direction on a project idea that has > realistic procedures and obviously something that is able to be done if > the equipment or materials are available. > > I appreciate your time to read these questions. > > If you can suggest a direction or a source--Thank YOU. > > Jack Massie, Research Teacher > Omak High School > Box 833 > Omak, WA 509 826-4730 > > home e-mail jdsm@televar.com > school e-mail jmassie@omaksd.wednet.edu > ========================================================================= Date: Mon, 10 Jan 2000 15:15:26 +0800 Reply-To: Heng Jian Sender: NEMA Discussion List From: Heng Jian Subject: Control of bark beetle by entomopathogenic nematodes Comments: To: =?gb2312?B?z9+z5rf+zvHG9w==?= MIME-version: 1.0 Content-type: multipart/alternative; boundary="----=_NextPart_000_0009_01BF5B7D.85BF2740" This is a multi-part message in MIME format. ------=_NextPart_000_0009_01BF5B7D.85BF2740 Content-Type: text/plain; charset="gb2312" Content-Transfer-Encoding: base64 RGVhciBTaXIsDQpJIHdvdWxkIGFwcHJlY2lhdGUgeW91IHZlcnkgbXVjaCBpZiB5b3UgY291bG Qg dGVsbCBtZSBhbnkgaW5mb3JtYXRpb24gYWJvdXQgdXNpbmcgZW50b21vcGF0aG9nZW5pYyBuZW 1h dG9kZSBvciBhbnkgb3RoZXIgYmlvbG9naWNhbCBjb250cm9sIGFnZW50cyBmb3Igc3VjY2Vzc2 Z1 bCBjb250cm9sIG9mIGJhcmsgYmVldGxlIChib3RoIFNjb3BseXR1cyBhbmQgRGVuZHJvY3Rvbn Vz KQ0KDQpIZW5nIEppYW4NCg0KSW5zdGl0dXRlIG9mIEJpb2xvZ2ljYWwgY29udHJvbA0KQ2hpbm Vz ZSBBY2FkZW15IG9mIEFncmljdWx0dXJhbCBTY2llbmNlcw0KQmVpamluZyAxMDAwODEsIENoaW 5h DQoNCkVtYWlsOiBoZW5namlhbkAyNjMubmV0DQpQaG9uZTogODYtMTAtNjg5MTk1NjINCkZheD og ODYtMTAtNjg5NzUxMDENCg== ------=_NextPart_000_0009_01BF5B7D.85BF2740 Content-Type: text/html; charset="gb2312" Content-Transfer-Encoding: base64 PCFET0NUWVBFIEhUTUwgUFVCTElDICItLy9XM0MvL0RURCBIVE1MIDQuMCBUcmFuc2l0aW9uYW wv L0VOIj4NCjxIVE1MPjxIRUFEPg0KPE1FVEEgY29udGVudD0idGV4dC9odG1sOyBjaGFyc2V0PW di MjMxMiIgaHR0cC1lcXVpdj1Db250ZW50LVR5cGU+DQo8TUVUQSBjb250ZW50PSJNU0hUTUwgNS 4w MC4yMDE0LjIxMCIgbmFtZT1HRU5FUkFUT1I+DQo8U1RZTEU+PC9TVFlMRT4NCjwvSEVBRD4NCj xC T0RZIGJnQ29sb3I9I2ZmZmZmZj4NCjxESVY+PEZPTlQgY29sb3I9IzAwMDAwMCBzaXplPTI+RG Vh ciBTaXIsPC9GT05UPjwvRElWPg0KPERJVj48Rk9OVCBjb2xvcj0jMDAwMDAwIHNpemU9Mj5JIH dv dWxkIGFwcHJlY2lhdGUgeW91IHZlcnkgbXVjaCBpZiB5b3UgY291bGQgDQp0ZWxsIG1lIGFueS Zu YnNwO2luZm9ybWF0aW9uIGFib3V0IHVzaW5nIGVudG9tb3BhdGhvZ2VuaWMgbmVtYXRvZGUgb3 Ig YW55IG90aGVyIA0KYmlvbG9naWNhbCBjb250cm9sIGFnZW50cyBmb3Igc3VjY2Vzc2Z1bCBjb2 50 cm9sIG9mIGJhcmsgYmVldGxlIChib3RoIFNjb3BseXR1cyANCmFuZCBEZW5kcm9jdG9udXMpPC 9G T05UPjwvRElWPg0KPERJVj4mbmJzcDs8L0RJVj4NCjxESVY+PEZPTlQgY29sb3I9IzAwMDAwMC Bz aXplPTI+SGVuZyBKaWFuPC9GT05UPjwvRElWPg0KPERJVj4mbmJzcDs8L0RJVj4NCjxESVY+PE ZP TlQgY29sb3I9IzAwMDAwMCBzaXplPTI+SW5zdGl0dXRlIG9mIEJpb2xvZ2ljYWwgY29udHJvbD xC Uj5DaGluZXNlIA0KQWNhZGVteSBvZiBBZ3JpY3VsdHVyYWwgU2NpZW5jZXM8QlI+QmVpamluZy Ax MDAwODEsIENoaW5hPC9GT05UPjwvRElWPg0KPERJVj4mbmJzcDs8L0RJVj4NCjxESVY+PEZPTl Qg Y29sb3I9IzAwMDAwMCBzaXplPTI+RW1haWw6IDxBIA0KaHJlZj0ibWFpbHRvOmhlbmdqaWFuQD I2 My5uZXQiPmhlbmdqaWFuQDI2My5uZXQ8L0E+PEJSPlBob25lOiANCjg2LTEwLTY4OTE5NTYyPE JS PkZheDogODYtMTAtNjg5NzUxMDE8L0ZPTlQ+PC9ESVY+PC9CT0RZPjwvSFRNTD4NCg== ------=_NextPart_000_0009_01BF5B7D.85BF2740-- ========================================================================= Date: Wed, 12 Jan 2000 11:22:05 -0600 Reply-To: dr1@plantpath.wisc.edu Sender: NEMA Discussion List From: DORITH ROTENBERG Organization: University of Wisconsin Subject: this is just a test Greetings fellow nematode-allies: The purpose of this short note is to test whether or not my lab mate, Jinya Qiu, successfully subscribed as a new member to the nema-list discussion group. Thanks. -Dorit Dorith Rotenberg UW-Madison Department of Plant Pathology 1630 Linden Dr. Madison, WI 53706 PH: 608-262-0061 dr1@plantpath.wisc.edu Dorith Rotenberg University of Wisconsin-Madison Dept. of Plant Pathology 1630 Linden Dr. Madison, WI 53706 (608)-263-2094 ========================================================================= Date: Thu, 13 Jan 2000 11:18:36 +0530 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Rajan Subject: Photos MIME-version: 1.0 Content-type: multipart/alternative; boundary="----=_NextPart_000_0033_01BF5DB7.EF7E66A0" This is a multi-part message in MIME format. ------=_NextPart_000_0033_01BF5DB7.EF7E66A0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Hello, As a teaching aid I wish to use multimedia. I do not have scanned = photographs of nematodes and field symptoms they cause. May I request = you scann and send these photographs through E-mail so that they are = directly used for teaching Nematology to trainees and students. Thanks = a lot for the favour. With regards, Rajan, National Bureau of Plant Genetic Resources, New Delhi 110 012, India ------=_NextPart_000_0033_01BF5DB7.EF7E66A0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
Hello,
As a teaching aid I wish to use = multimedia. I do=20 not have scanned photographs of nematodes and field symptoms they cause. = May I=20 request you scann and send these photographs through E-mail so that they = are=20 directly used for teaching Nematology  to trainees and students. = Thanks a=20 lot for the favour.
With regards,
Rajan,
National Bureau of Plant Genetic=20 Resources,
New Delhi 110 012,
India
------=_NextPart_000_0033_01BF5DB7.EF7E66A0-- ========================================================================= Date: Thu, 13 Jan 2000 09:38:04 -0200 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: "Leandro G. Freitas" Subject: Re: Photos In-Reply-To: <01bf5d89$d5c62aa0$1a01a8c0@quarantine.nbpgr.delhi.nic.in> MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" Content-transfer-encoding: quoted-printable Hi to all nematologists, I teach plant nematology at the University of Vi=E7osa, Brazil, and I als= o use multimedia in my classes. I would like to have more pictures of nematodes, symptoms and other related subjects, such as management methods. If you are going to send some pictures to Dr. Rajan, please send them to me too, at "leandro@mail.ufv.br". I appreciate the collaboration, Best regards, Leandro Grassi de Freitas, PhD. Departamento de Fitopatologia Universidade Federal de Vi=E7osa Vi=E7osa, MG 36571-000 Brazil ========================================================================= Date: Thu, 13 Jan 2000 21:46:42 +0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Paul Speijer Subject: Re: Photos In-Reply-To: <200001131135.JAA08658@mail.cpd.ufv.br> MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" Content-transfer-encoding: quoted-printable The Nematological Society of Southern Africa had produced an excellent slide serie a few years ago. Just check if that is still available. Regards, Paul At 09:38 AM 1/13/00 -0200, you wrote: >Hi to all nematologists, > > I teach plant nematology at the University of Vi=E7osa, Brazil, and I= also >use multimedia in my classes. I would like to have more pictures of >nematodes, symptoms and other related subjects, such as management >methods. > If you are going to send some pictures to Dr. Rajan, please send them to >me too, at "leandro@mail.ufv.br". > I appreciate the collaboration, > Best regards, > > > Leandro Grassi de Freitas, PhD. > Departamento de Fitopatologia > Universidade Federal de Vi=E7osa > Vi=E7osa, MG 36571-000 > Brazil > > International Institute of Tropical Agriculture (IITA),=20 Eastern and Southern Africa Regional Centre (ESARC),=20 P.O.Box 7878, Kampala, Uganda. Tel:00256 (Uganda) 075-787802 (office) 077-425576 (mobile) 041-223460 (Kampala office) 041-236918 (home phone/fax) Fax:041-223459 (Kampala office) ========================================================================= Date: Sun, 16 Jan 2000 12:28:36 +0100 Reply-To: ruffoo11@worldonline.es Sender: NEMA Discussion List From: "D.Ruffo" Subject: Pratylenchus coffeae. MIME-version: 1.0 Content-type: multipart/alternative; boundary="------------6260333D63C83CC384C75FF3" --------------6260333D63C83CC384C75FF3 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Hi all, I am a new pride lover of these beings. Anybody could give me some information about Pratylenchus coffeae infections in banana fields,would thank you any data regarding speed of spread ,temperatures and soil conditions that favors his propagation.Above all i'm very interested in knowing the best method to recount and collect them. David. --------------6260333D63C83CC384C75FF3 Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit Hi all,

I am a new pride lover of these beings.
Anybody could give me some information about Pratylenchus coffeae infections in banana fields,would thank you any data regarding speed of spread ,temperatures and soil conditions that favors his propagation.Above all i'm very interested in knowing the best method to recount and collect them.

David. --------------6260333D63C83CC384C75FF3-- ========================================================================= Date: Mon, 17 Jan 2000 00:43:11 -0100 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Francisco Pinto Subject: Fw: Articles of Fedorko, A. , 1977 MIME-version: 1.0 Content-type: MULTIPART/ALTERNATIVE; BOUNDARY="Boundary_[ID_5oMXcXRPUsWaZworf3QRsg]" Esta é uma mensagem com várias partes em formato MIME. --Boundary_[ID_5oMXcXRPUsWaZworf3QRsg] Content-type: text/plain; charset=iso-8859-1 ----- Original Message ----- From: Francisco Pinto To: Nema-L Sent: Monday, January 17, 2000 12:39 AM Subject: Articles of Fedorko, A. , 1977 Hello Could any one tell me how to get a copy of the papers of Fedorko A. regarding the effects os herbicides and insecticides on Nematodes? Thank You francpinto@mail.telepac.pt --Boundary_[ID_5oMXcXRPUsWaZworf3QRsg] Content-type: text/html; charset=iso-8859-1

 
----- Original Message -----
To: Nema-L
Sent: Monday, January 17, 2000 12:39 AM
Subject: Articles of Fedorko, A. , 1977

Hello
 
Could any one tell me how to get a copy of the papers of Fedorko A. regarding the effects  os herbicides and insecticides on Nematodes?
 
Thank You
 
--Boundary_[ID_5oMXcXRPUsWaZworf3QRsg]-- ========================================================================= Date: Tue, 18 Jan 2000 10:59:01 -0200 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: "Leandro G. Freitas" Subject: Re: Photos In-Reply-To: <3.0.1.32.20000113214642.00fdab30@imul.com> MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" Content-transfer-encoding: quoted-printable Thanks Paul At 21:46 13/01/00 +0100, you wrote: >The Nematological Society of Southern Africa had produced an excellent >slide serie a few years ago. Just check if that is still available. > >Regards, > Paul > > >At 09:38 AM 1/13/00 -0200, you wrote: >>Hi to all nematologists, >> >> I teach plant nematology at the University of Vi=E7osa, Brazil, and >> I= also >>use multimedia in my classes. I would like to have more pictures of >>nematodes, symptoms and other related subjects, such as management= methods. >> If you are going to send some pictures to Dr. Rajan, please send them >> to >>me too, at "leandro@mail.ufv.br". >> I appreciate the collaboration, >> Best regards, >> >> >> Leandro Grassi de Freitas, PhD. >> Departamento de Fitopatologia >> Universidade Federal de Vi=E7osa >> Vi=E7osa, MG 36571-000 >> Brazil >> >> >International Institute of Tropical Agriculture (IITA),=20 >Eastern and Southern Africa Regional Centre (ESARC),=20 >P.O.Box 7878, Kampala, Uganda. > >Tel:00256 (Uganda) > 075-787802 (office) > 077-425576 (mobile) > 041-223460 (Kampala office) > 041-236918 (home phone/fax) > >Fax:041-223459 (Kampala office) >=20 ========================================================================= Date: Tue, 18 Jan 2000 12:13:04 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: john solomon Subject: Help me out. Comments: To: nema-l@unl.edu MIME-version: 1.0 Content-type: text/plain; charset=us-ascii Hello Everybody, I am a research student from India who need help from you all to get necessary information for the following queries. 1. Where will I get details about Plant parasitic nematode in the internet. Please suggest some web-sites for details. 2. Can you give me any book name which deals with this topic. I hope somebody will help me out to get the details. Thank You, Reply to, Nirmala Grace, No. 2 ,15th Street, R.E.C, Trichy - 620015. Tamil Nadu, India. E-Mail Address : NATADA@rect.ernet.in or : john_solojames@yahoo.com Yours truly, Nirmala Grace. __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com ========================================================================= Date: Tue, 18 Jan 2000 16:06:56 -0500 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: "Flack, Earl" Subject: Re: Help me out. MIME-version: 1.0 Content-type: text/plain; charset="iso-8859-1" http://pppweb.clemson.edu/NematodeSites.html I hope that this helps. Earl A. Flack Bureau of Plant Industry Pennsylvania Department of Agriculture 2301 N. Cameron St. Harrisburg, Pa 17110-9408 e-mail: eflack@state.pa.us phone: (717) 705-4343 fax: (717) 705-6518 -----Original Message----- From: john solomon [mailto:john_solojames@YAHOO.COM] Sent: Tuesday, January 18, 2000 3:13 PM To: NEMA-L@crcvms.unl.edu Subject: Help me out. Hello Everybody, I am a research student from India who need help from you all to get necessary information for the following queries. 1. Where will I get details about Plant parasitic nematode in the internet. Please suggest some web-sites for details. 2. Can you give me any book name which deals with this topic. I hope somebody will help me out to get the details. Thank You, Reply to, Nirmala Grace, No. 2 ,15th Street, R.E.C, Trichy - 620015. Tamil Nadu, India. E-Mail Address : NATADA@rect.ernet.in or : john_solojames@yahoo.com Yours truly, Nirmala Grace. __________________________________________________ Do You Yahoo!? Talk to your friends online with Yahoo! Messenger. http://im.yahoo.com ========================================================================= Date: Wed, 19 Jan 2000 00:06:36 -0800 Reply-To: wambui@mailcity.com Sender: NEMA Discussion List From: catherine wambui Organization: MailCity (http://www.mailcity.lycos.com:80) Subject: Remove from list MIME-version: 1.0 Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit Kindly remove me from the distribution list with immediate effect. Catherine LYCOShop. Thousands of products! One location! http://shop.lycos.com/ ========================================================================= Date: Thu, 20 Jan 2000 12:34:12 -0600 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Allen Szalanski Subject: nematode of the week MIME-version: 1.0 Content-type: text/enriched; charset=US-ASCII Content-transfer-encoding: 7BIT 0100,0100,0100A "nematode of the week" page has been added to the Society of Nematologists WWW site: http://www.ianr.unl.edu/son/ The purpose of this page is to provide information on interesting and unusual nematodes. This week its Enchodelus analatus, collected from the Canadian arctic. Allen Szalanski ********************************************************** Allen L. Szalanski Research Assistant Professor Department of Plant Pathology University of Nebraska-Lincoln 406 Plant Science Hall Lincoln, NE 68583-0722 Phone: 402-472-3167 Email: aszalans@unlserve.unl.edu http://ianrwww.unl.edu/ianr/plntpath/nematode/aszalans.htm **********************************************************