Molecular Identification of Plant Parasitic Nematodes

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Amplification of rDNA ITS1 region, and portions of the 18S and 5.8S genes using primers rDNA2 and rDNA1.58S  

Genus	species	Amplicon  size (bp)					RFLP
Criconemella		600
Heterodera	geottingiana  zeae  glycines  schachtii  trifolii  cruciferae	800		Hha I  Hha I  Hha I  Hha I  Hha I  Hha I		Hinf I  Hinf I  Hinf I  Hinf I  Hinf I  Hinf I
Globodera	pallida  rostochiensis  tabacum	750	Hae III  Hae III  Hae III	Hha I  Hha I  Hha I	Hinc II  Hinc II  Hinc II	Hinf I  Hinf I  Hinf I
Heteroderamani		800
Meloidogyne		450
Pratylenchus		650
Tylenchus		600
Helicotylenchus		900
Tylenchorhynchus		600
Hoplolaimus		800
Belonolaimus		700
Xiphinema		900
Longidorus		1200
Geocenamus		500
Quinisulcius		500
Nacobbus		500
Rotylenchus		900
Helicotylenchus		850
Scutellonema		900
Aorolaimus		900

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