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Cysts
Measurements (n = 10). Length (excluding neck and vulval cone): 635
um (568-728 um); width: 466 um (364-570 um); vulval cone: 79.6 um (70-84
um); depth of underbridge: 71.6 um (64-80 um). Lemon-shaped with distinct
neck and vulval cone. Neck usually twisted. Colour changes
from creamy to tan into brown during maturation. Thick subcrystalline
layer which sticks firmly to the cyst. Cuticle with zig-zag pattern
and scattered punctation. Sometimes small egg sac present without
any content.
Cyst cone ambifenestrate. Fenestra measurements
(n = 15). Length: 46.6 um (39-55um); width: 34.1 um (30-40 um); vulval
slit: 44.7 um (39-55 um). Vulval bridge 5-10 um wide; underbridge
(n = 9): 40-60 um long and 6-10 um wide. Bullae present, well developed.
Second-stage juveniles
Measurements (n = 100). L: 462 um (417-512 um); a: 24.4 (22.0-27.6);
c: 8.8 (7.5-11.4); spear: 25 um (24-26 um); head-end to median bulb valve:
72 um (62-82 um); head-end to excretory pore: 19 um (18-20 um); anal body
width: 13 um (11-15 um).
Body cylindrical, gently curved with a conical
tail. Lateral field with four incisures, not aerolated. Lip
region rounded, offset with two head annules. Spear knobs well developed,
rounded posteriorly, concave anteriorly. Dorsal oesophageal gland
opening 5-6 um behind the spear knobs. Median oesophageal bulb valve
plates prominent. Excretory pore located 93-120 um from the anterior
end. Heminzonid two body-annules wide, just in front of the excretory
pore. Oesophageal-intestine junction obscure. Oesophageal gland
lobe elongated, extending about 40% of the body-length and filling most
often half of the body cavity. Dorsal gland nucleus prominent, sub-ventral
gland nuclei more posterior and smaller. Genital primordium with
two nuclei located at 51-60% of the body length. Tail 51.8 um (41-60
um) long, with rounded terminus. Hyaline terminal section 28.5 um
(22-33 um) long and on the average 1.1 times the spear length. Phasmids
very small, located at the anterior third of the tail.
Eggs
Measurements (n = 30). Length: 102.6 um (98-115 um); width: 46.9
um (44-51 um); L/B 2.1 (1.9-2.4).
Male
Measurements (n = 25). L: 1322 um (1140-1470 um); a: 58.7 (49-69);
width at excretory pore: 21.6 um (21-26 um); spear 28.8 um (28-30 um);
head-end to median bulb valve: 97.7 um (83-116 um); head-tip to excretory
pore: 157.7 um (131-187 um); anal body width: 15.5 um (14-18 um).
Cuticle annulated. Lateral field with four incisures, appears
not aerolated. Lip region rounded, offset with four or five annules.
Spear knobs rounded. Dorsal oesophageal gland opening 5 um posterior
to spear knobs. Median oesophageal bulb ellipsoid; valve plates prominent,
situated in the posterior third part of the bulb. Excretory pore
131-187 um from anterior end. Hemizonid about 2 annules wide, 5-13
annules anterior to excretory pore.
Hemizonion not observed. Oesophageal-intestine
junction usually about level of excretory pore. Oesophageal gland
lobes filling nearly the whole body cavity. Nucleus of the dorsal
gland bigger than the two subventral nuclei. Testis one. Spicules
curved, 32-39 um long (N = 4) with bidentate tips. Cuticle protruding
around the cloacal aperture. Gubernaculum a simple rod, 10-11 um
long. Cuticle of tail tip ventrally thickened, forming a small cap.
Tail usually twisted. Phasmids not observed.
(Description- Gerber and Maas, 1982)
DNA Sequences Obtained
| Specimen: | Collected: |
| KS Alfalfa-3 | Lakin, Kansas |
| KS Alfalfa-1 | Lakin, Kansas |
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