Date sent: Fri, 11 Feb 2000 07:51:34 -0600 From: "L-Soft list server at UNIVERSITY OF NE - LINCOLN (1.8d)" Subject: File: "NEMA-L LOG9707" To: Allen Szalanski ========================================================================= Date: Tue, 1 Jul 1997 16:59:04 +0000 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: John Browne Subject: Advise Needed Comments: To: nema-l@unl.edu MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT I am currently trying to culture a slow growing Panagrolaimus species on OP50-1 plates and have encountered problems with yeast contamination. The standard alkaline hypochlorite cleaning has proved unsuccessful as the eggs hatchbut fail to develop into fertile adults. The culture is so slow growing that it will not out grow the contamination. All advise with regards the problem would be welcomed. Yours Thankfully John browne NUI Maynooth Ireland ========================================================================= Date: Tue, 1 Jul 1997 11:35:34 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Jay Burr Subject: Re: Advise Needed In-Reply-To: <01IKQ9X4V12Q00CHOU@ailm.may.ie> MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7bit Dear John, With C. elegans we prefer to let them self-clean. An individual is transferred by pick to a food plate, allowed to crawl through food for an hour, then transfered to a new food plate. This allows spores to be wiped off and expelled from the gut. With Panagrolaimus you might want to experiment with longer times and several transfers. - Jay - > > I am currently trying to culture a slow growing Panagrolaimus species > on OP50-1 plates and have encountered problems with yeast contamination. > The standard alkaline hypochlorite cleaning has proved unsuccessful > as the eggs hatchbut fail to develop into fertile adults. The culture > is so slow growing that it will not out grow the contamination. All > advise with regards the problem would be welcomed. > > Yours Thankfully > > John browne > NUI Maynooth > Ireland > ========================================================================= Date: Wed, 2 Jul 1997 10:11:56 +0000 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Mark Blaxter Subject: Reminder: Parasitic Helminths Meeting Comments: To: schistosoma@dl.ac.uk, nema-l@unl.edu, parasitology@dl.ac.uk MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Reminder! Parasitic Helminths from Genomes to Vaccines Edinburgh, 6-9 September 1997 This meeting is the first of what is hoped will be a new series of annual or biannual research meetings in Edinburgh. "Parasitic Helminths from Genomes to Vaccines" is intended to fill an important gap in the current round of regular conferences. There have been recent major advances in helminth research in the fields of gene discovery and genomics, biochemistry and physiology (e.g. neurobiology and receptors), and the immunology of host-parasite interactions. The value of a helminth-specific meeting (rather than a general parasitology meeting such as Woods Hole) has been demonstrated by recent Keystone symposia on Molecular Helminthology. This meeting hopes to make such helminth-specific gatherings more frequent and more accessible, especially at the student and postdoc levels. We hope that there will be a fruitful interaction between researchers from the C. elegans field and the many branches of molecular, biochemical and immunological parasitology. Information on the meeting is updated regularly on the World Wide Web at http://helios.bto.ed.ac.uk/mbx/blaxter/PHfG2V/ParasiticHelminths.html All conference enquiries should be addressed to registration forms should be requested _urgently_ from marnie.ferguson@ed.ac.uk or by mail/phone/fax from Parasitic Helminths Conference UnivEd Technologies Limited, UnivEd Training and Conference Centre, 11 South College Street, Edinburgh EH8 9AA, Scotland UK Tel: +44 (0)131 650 9020 Fax: +44 (0)131 650 9019 ========================================================================= Date: Thu, 3 Jul 1997 14:19:00 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: "Safia Siddiqi (CABI)" Subject: Crop Protection Compendium Comments: To: nema MIME-version: 1.0 Content-type: TEXT/PLAIN Subscribers to this list may be interested to know that CAB International has announced the publication of the Crop Protection Compendium, Module 1. We are offering a free trial CD-ROM, for Windows. This is a walk through the Compendium which demonstrates the diagnostic and illustrative potential of the CD-ROM by narrowing down through country, crop and symptom. To obtain a trial disk email S.Harris@CABI.org The Crop Protection Compendium CD-ROM is available from CAB INTERNATIONAL at US Dollars 2000 for developed countries and at US Dollars 500 for developing countries, thanks to funding from members of the Crop Protection Compendium Development Consortium. Module 1 provides easy to use, instant access to detailed, illustrated data on CD-ROM for 1000 major pests and their natural enemies, many of global importance with specific emphasis on South-East Asia and Pacific. It includes diseases and weeds,150 crops of the world and 150 countries of the world. In addition, there are illustrated diagnostic keys, including CABIKEY; an open architecture, with links to the internet and technical support pages on PEST CABWeb (http://pest.cabweb.org/cpc/cpchp.htm); a bibliographic database of more than 50,000 references on pest management, with abstracts; corporate or personal electronic notepads for keeping and sharing notes; and an interactive glossary. The Crop Protection Compendium CD-ROM is a simple tool for pest diagnosis with powerful search features and the flexibility to narrow down by country, crop and symptom. If you would like further details, please see the Crop Protection Compendium pages on PEST CABWeb, at http://pest.cabweb.org/cpc/cpchp.htm, or contact CABI at http://www.cabi.org/ ========================================================================= Date: Wed, 9 Jul 1997 13:25:56 +0000 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Cesar Subject: nematicide test in vitro Comments: To: nema-l@unl.edu MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT Dear Nemas : I am looking for information about nematicide test in vitro. Can anyone tell me about any literature have this information? Thanks Cesar Ornat Departament Patologia vegetal IRTA Ctra de Cabrils s/n 08348 CABRILS BARCELONA SPAIN Tf. 3 93 750 75 11 tmp2ie01@cabrils.irta.es meloidogyne@geocities.com http://www.geocities.com/rainforest/7473 ========================================================================= Date: Wed, 9 Jul 1997 08:34:53 -0400 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Grover Smart Subject: Re: nematicide test in vitro MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dr. A. C. Tarjan did in vitro testing of nematocides several years ago. >Dear Nemas : > >I am looking for information about nematicide test in vitro. > >Can anyone tell me about any literature have this information? > > >Thanks >Cesar Ornat > >Departament Patologia vegetal >IRTA >Ctra de Cabrils s/n >08348 CABRILS >BARCELONA >SPAIN > >Tf. 3 93 750 75 11 > >tmp2ie01@cabrils.irta.es >meloidogyne@geocities.com > >http://www.geocities.com/rainforest/7473 > Grover Smart GCS@GNV.IFAS.UFL.EDU ========================================================================= Date: Fri, 11 Jul 1997 21:49:24 +0300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Michal Brzeski Organization: Instytut Warzywnictwa Subject: nematodes in forest nurseries MIME-version: 1.0 Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit Dear Colleagues, I am now at process of getting some money for survey of forest nurseries in Poland. First analyses show we sometime may have problems with Paratrichodorus pachydermus, may be also Cephalenchus hexalineatus as this later may exceed 2000 nematodes in 100 cc of soil. However, my sponsor wishes to get also some practical advice and not only results of survey. Therefore, I would much appreciate any information how it is in other countries and what are the practical steps taken to decrease the damage done by these nemas to seedlings. Best regards to all, Michal W. Brzeski ========================================================================= Date: Fri, 11 Jul 1997 13:58:10 -0700 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Kaiji Hu Subject: Re: nematicide test in vitro In-Reply-To: from <"Cesar"@Jul> MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7bit > Hello Cesar: There are hundreds of papers regarding the in vitro test of nematicides in a wide range of publications. A few of them are: Ann. appl. Biol. 1975, Vol 79: 329-341. Revue Nematol. 1988, Vol 11(4):399-404 Nematologica 1993, Vol 39: 521-535. Good luck! Kaiji Hu Dept. Biological Sciences Simon Fraser University Vancouver, BC, Canada V5A 1S6 > Dear Nemas : > > I am looking for information about nematicide test in vitro. > > Can anyone tell me about any literature have this information? > > > Thanks > Cesar Ornat > > Departament Patologia vegetal > IRTA > Ctra de Cabrils s/n > 08348 CABRILS > BARCELONA > SPAIN > > Tf. 3 93 750 75 11 > > tmp2ie01@cabrils.irta.es > meloidogyne@geocities.com > > http://www.geocities.com/rainforest/7473 > ========================================================================= Date: Tue, 15 Jul 1997 08:32:02 +0000 Reply-To: rfdavis@uga.cc.uga.edu Sender: NEMA Discussion List Comments: Authenticated sender is From: "Richard F. Davis" Organization: University of Georgia Subject: Symposium Topics? MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT In 1998, the American Phytopathological Society and the Entomological Society of America will meet jointly in Las Vegas. At that meeting, we (the APS Nematology Committee and various ESA subsections) hope to have a joint session focussing on nematode/insect interrelationships. What topics can you think of that would be appropriate? One topic might be the relationships in the pine wood nematode complex. Other topics that come to mind are interesting from both a nematology and entomology viewpoint (such as entomopathogenic nematodes, and non-pathogenic entomogenous nematodes), but they don't have much to do with diseases of plants. And that is the problem. If you have any ideas about possible topics, especially topics that relate to diseases of plants, please let me know. Thanks. RFD ********************************************************* Richard F. Davis Internet:RFDAVIS@UGA.CC.UGA.EDU University of Georgia Phone: 706-542-9146 Extension Plant Pathology Fax: 706-542-4102 2106 Miller Plant Sciences Bldg. Athens, GA 30602-7274 ========================================================================= Date: Tue, 15 Jul 1997 10:07:02 -0700 Reply-To: mullinp@edenbio.com Sender: NEMA Discussion List From: Peter Mullin Organization: EDEN Bioscience Corporation Subject: Re: nematodes in forest nurseries MIME-version: 1.0 Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit Michal, In the Pacific Northwest U.S, we frequently see damage to forest nursery seedlings (most often Pseudotsuga menziesii, but also other conifers and hardwoods) caused by Pratylenchus penetrans, occasionally P. crenatus, Xiphinema bakeri, and X. americanum (less commonly), among others. I don't have any reliable damage thresholds for these nematodes, nor for Cephalenchus, which I see frequently but have never been able to correlate with appreciable damage to seedling roots. We basically do pre-plant soil surveys for nurseries, and make recommendations for or against preplant fumigation with methyl bromide, metam sodium (Vapam), or dichlorpropene (Telone) or for cultural control methods such as tillage or disking of soils during hot weahter, depending on what and how manyt nematodes are present. In some cases, postplant nematicides such as fenamiphos or oxamyl can be effective, but they are often not economic for the grower. Hope this helps. Peter Mullin Diagnostic Laboratory EDEN Bioscience 11816 North Creek Parkway North Bothell, WA 98011 (800) 635-6866 ========================================================================= Date: Tue, 15 Jul 1997 21:35:15 +0300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Michal Brzeski Organization: Instytut Warzywnictwa Subject: Nematodes in forest nurseries MIME-version: 1.0 Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7bit Dear Colleagues, I am posting this request again as Mail Delivery System informed me that it did not reached long list of Nema-L subscribers. Those that already got this message are kindly asked for comments. I am near to get some funds for survey of nematodes in forest nurseries, although my sponsor wishes to get not only the list of species present, but also some practical advice. From what I have seen till now we probably have some problems with Paratrichodorus pachydermus, Rotylenchus uniformis, possibly also Cephalenchus hexalineatus. This latter sometimes exceed 2000 nematodes in 100 cc of soil. Therefore, I would much appreciate information how is the situation in other countries and what are practical steps taken to limit the damage done by nematodes. Since I did not got any reaction on my first posting, I assume the question may not be widely recognized and of more general interest, thus comments to the list would be appropriate. Thanking you in advance for help and comments, Best regards to all, Michal W. Brzeski ========================================================================= Date: Fri, 25 Jul 1997 16:21:30 -0800 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: "Xiang Huang, UCR Nematology" Subject: Medium for tomato roots Comments: To: nema-l@unl.edu MIME-version: 1.0 Content-type: text/plain; charset="us-ascii" Dear friends, Does anybody know about an in vitro culture medium that supports tomato root explants well and has lower salt level than Gamborg's B5? I appreciate your help very much. XH ------------------------------------------------------- Xiang Huang * Department of Nematology * Tel.: 909/787-4586 University of California * FAX: 909/787-3719 Riverside, CA 92521-0415 * xhuang@ucrac1.ucr.edu ------------------------------------------------------- ========================================================================= Date: Mon, 28 Jul 1997 08:34:00 +1300 Reply-To: NEMA Discussion List Sender: NEMA Discussion List From: Gregor Yeates Subject: Medium for tomato roots -Reply MIME-version: 1.0 Content-type: TEXT/PLAIN; Charset=US-ASCII Content-transfer-encoding: 7BIT Gregor Yeates is overseas (USA, UK). He will be back at work on 1 September. For urgent matters Landcare Research (PN) reception has contact details. ========================================================================= Date: Wed, 30 Jul 1997 23:02:09 -0230 Reply-To: Ian King Sender: NEMA Discussion List From: Ian King Subject: Extraction methods MIME-version: 1.0 Content-type: TEXT/PLAIN; charset=US-ASCII I'm doing a survey of the nematode fauna of a region and my soil samples are relatively large (greater than 300 ml). I'm considering a sieving/centrifugation technique after Jenkins (1964) in Southey (1985). It basically (I'll try to be brief as most of you are probably familiar with this) involves washing the soil through a 840 um sieve into a bucket and making up to 6 litres, stirring and pouring supernatant through 53 um sieve (these first steps are repeated). The sievings are centrifuged at 1750 rpm for 4-5 minutes. The supernatant is replaced with a sucrose solution with specific gravity of 1.15 and centrifuged again for 30-60 seconds. The supernatant is then poured through a 53um (or less) sieve and the sievings washed and collected in a beaker. One change: the smallest sieve I have is 125um so unless I can get a 53um one, I was considering making one from Nitex which has a mesh size of 15um and using it both times a 53um sieve is to be used (too small?) Any thoughts on the above method (whether I should change part of it or abandon it in favour of something else; and on the use of the Nitex filter) would be greatly appreciated. Thanks in advance for any advice. Ian Wm. King Dept. of Biology Memorial University of Newfoundland St. John's, NF, Canada