>From TOMMINEN@LadyBird.Helsinki.FI Wed Jan 8 07:27:13 1997 Received: from kantti.helsinki.fi (kantti-fddi.Helsinki.FI) by crcnis1.unl.edu with SMTP id AA11937 (5.65c/IDA-1.4.4 for ); Wed, 8 Jan 1997 07:27:13 -0600 Received: from millipede.helsinki.fi (millipede.helsinki.fi [128.214.94.94]) by kantti.helsinki.fi (8.8.3/8.8.2) with ESMTP id PAA25939 for ; Wed, 8 Jan 1997 15:24:12 +0200 (EET) Received: from MILLIPEDE/SpoolDir by millipede.helsinki.fi (Mercury 1.21); 8 Jan 97 15:27:15 EET Received: from SpoolDir by MILLIPEDE (Mercury 1.21); 8 Jan 97 15:26:53 EET From: "Jyrki Tomminen" Organization: University of Helsinki To: nema-l@unl.edu Date: Wed, 8 Jan 1997 15:26:35 EET DST Subject: questions Reply-To: Tomminen@millipede.Helsinki.FI X-Confirm-Reading-To: Tomminen@Viikki.Helsinki.Fi X-Pmrqc: 1 Priority: normal X-Mailer: Pegasus Mail v3.22 Message-Id: <2D0A52C6C7D@millipede.helsinki.fi> Thanks again for everybody that responded to my previous questions (not very many but few anyway). Hapyy new year to all of you, too. Here follows a couple of questions: 1. We in our laboratory are considering purchasing MEKU (Erich Pollahne) Soil Sample Extractor (with MEKU High Pressure Rinsing Pump) for separating pcn cysts from soil samples. Anyone has any experience with this machinery or anything that would be comparable to that. I should be easy and quick processing of the samples with no technical flaws? A comment from a representative of the company will be ignored... 2. Does anyone know if there'd already be PCR primers commercially available for identificating potato cyst nematodes (rostochiensis vs. pallida)? How about for Meloidogyne (chitwoodi/hapla/fallax)? Please all of you that really have something to say about these matters take a stand and speak! Thank you very much. - Jyrki - >From idl@ageri.sci.eg Mon Jan 13 16:54:21 1997 Received: from FRCU.EUN.EG by crcnis1.unl.edu with SMTP id AA05687 (5.65c/IDA-1.4.4 for ); Mon, 13 Jan 1997 23:11:37 -0600 Received: from ageri.sci.eg by FRCU.EUN.EG (PMDF V4.2-11 #3805) id <01IE70K6P1XS0014T0@FRCU.EUN.EG>; Tue, 14 Jan 1997 07:09:01 O Received: from ageri by ageri.sci.eg (SMI-8.6/SMI-SVR4) id OAA06154; Mon, 13 Jan 1997 14:54:21 +0200 Date: Mon, 13 Jan 1997 14:54:21 +0200 From: idl@ageri.sci.eg Subject: CHEF gel To: nema-l@unl.edu Message-Id: <199701131254.OAA06154@ageri.sci.eg> Mime-Version: 1.0 X-Mailer: Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7BIT X-Sender: idl@ageri.sci.eg Dear Nematologists: I need more information about using Pulsed Field Gel Electrophoresis with nematode. So, I will be very appreciated if anybody there give me more information about that or even guide me to the resources which I can use it to get these information. Thanks in advance. Nasser *********************************************************** Abd El-Nasser M. Elashry Agricultural Genetic Engineering Research Institute (AGERI) Agricultural Research Center (ARC) 9 Gamaa st., Giza 12619 Egypt Email idl@ageri.sci.eg *********************************************************** >From agAccess@davis.com Tue Jan 21 07:55:47 1997 Received: from pa.mother.com by crcnis1.unl.edu with SMTP id AA12092 (5.65c/IDA-1.4.4 for ); Tue, 21 Jan 1997 17:58:39 -0600 Received: from [207.104.33.3] (m2p76.dav.mother.com [207.104.33.76]) by pa.mother.com (8.8.4/8.8.0) with SMTP id PAA06382 for ; Tue, 21 Jan 1997 15:55:47 -0800 Date: Tue, 21 Jan 1997 15:55:47 -0800 X-Sender: agaccess@mother.com Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" To: nema-l@unl.edu From: agAccess@davis.com Subject: Economic Impact of nematodes, resistant cultivars Dear Nema-L, I'm researching the economic impact of nematodes on all major crops in the U.S. I'm also compiling information on nematode resistant cultivars. Any information you could provide in this direction would be greatly appreciated. Sincerely, Nathan Boone Research Director Agricola Partners ===================================================== | Agricola Partners :: Tel: 916-750-2264 | | 603 Fourth Street :: Fax: 916-756-7188 | | Davis, CA 95616 :: Email: Agricola@davis.com | | www: http://www.mother.com/agaccess/Agricola.html | |=====================================================| | Agricola Partners is dedicated to the agriculture | | and food industries with an emphasis on | | sustainable agriculture and organic farming. | ===================================================== >From dchitwoo@asrr.arsusda.gov Tue Jan 28 04:05:59 1997 Received: from asrr (asrr.arsusda.gov) by crcnis1.unl.edu with SMTP id AA07947 (5.65c/IDA-1.4.4 for ); Tue, 28 Jan 1997 08:06:37 -0600 Received: from dchitwoo.asrr.arsusda.gov ([198.77.169.126]) by asrr (5.x/SMI-SVR4) id AA20096; Tue, 28 Jan 1997 09:04:38 -0500 Message-Id: <2.2.32.19970128140559.006b8578@asrr.arsusda.gov> X-Sender: dchitwoo@asrr.arsusda.gov X-Mailer: Windows Eudora Pro Version 2.2 (32) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Tue, 28 Jan 1997 09:05:59 -0500 To: nema-l@unl.edu From: David Chitwood Subject: Position Vacancy-- USDA, ARS, Beltsville, Maryland POSITION VACANCY U.S. DEPARTMENT OF AGRICULTURE AGRICULTURAL RESEARCH SERVICE BELTSVILLE, MARYLAND ARS is seeking an interdisciplinary Research Molecular Biologist/Research Plant Pathologist/Zoologist, GS-401/434/ 410-11/12 in the Nematology Laboratory, Plant Sciences Institute, Beltsville Agricultural Research Center. Salary is commensurate with experience (GS-11: $38,330-$49,831 per annum; GS-12: $45,939-$59,725 per annum). The position involves planning and conducting independent and cooperative research primarily on the molecular biology and molecular genetics of plant-parasitic nematodes and host-parasite relationships. In addition to the basic educational requirements, applicants must demonstrate 1) knowledge of the principles, methods, techniques and procedures of molecular biology, and of plant pathology, genetics, plant nematology, or parasitology; 2) ability to use molecular techniques (including gene cloning, DNA sequencing, etc.) in research involving plant-parasitic nematode biology or nematode interactions with host plants; 3) knowledge of plant-parasitic nematodes and their relationship to plant diseases; and 4) ability to design, plan, and conduct research and publish results. For information on the research program and/or position, contact Dr. David J. Chitwood, 301-504-5660. For information on application procedures/forms, please call Veronica Olaaje for a copy of the announcement on 301-344-l014. Applications in response to this advertisement should be marked ARS-D7B-049/B-7-03. The U.S. Department of Agriculture is an Equal Opportunity Employer. Full applications must be postmarked by February 24. Greater details about the position and application procedures can be found on the USDA ARS Web Site at: http://www.ars.usda.gov/afm/hrd/d7b-049.htm David J. Chitwood Research Leader Nematology Laboratory, USDA Building 011A, Room 165B, BARC-West Beltsville, Maryland 20705 USA 301-504-8634 Fax: 301-504-5589 Internet: dchitwoo@asrr.arsusda.gov >From dtrudg@scri.sari.ac.uk Wed Jan 29 11:56:34 1997 Received: from caird.scri.sari.ac.uk by crcnis1.unl.edu with SMTP id AA08631 (5.65c/IDA-1.4.4 for ); Wed, 29 Jan 1997 05:59:08 -0600 Received: from law.scri.sari.ac.uk (law.scri.sari.ac.uk [134.36.144.25]) by caird.scri.sari.ac.uk (8.6.9/8.6.9) with ESMTP id LAA25754 for ; Wed, 29 Jan 1997 11:56:04 GMT Received: from LAW/MERCURY by law.scri.sari.ac.uk (Mercury 1.21); 29 Jan 97 11:56:49 GMT Received: from MERCURY by LAW (Mercury 1.21); 29 Jan 97 11:56:37 GMT From: "David Trudgill" To: nema-l@unl.edu Date: Wed, 29 Jan 1997 11:56:34 GMT Subject: Re: CHEF gel Return-Receipt-To: "David Trudgill" Priority: normal X-Mailer: Pegasus Mail for Windows (v2.42a) Message-Id: <23E88F11422@law.scri.sari.ac.uk> Dear Colleague, We tried pulse gel electophoresis on Meloidogyne chromosomes several years ago. If I remember correctly, we got a little bit of separation, but not much. Mireille Fargette, ORSTOM, Montpellier, may be able to remember more because she did the work. David Trudgill. >From xhuang@ucrac1.ucr.edu Mon Feb 3 07:19:45 1997 Received: from navel.ucr.edu by crcnis1.unl.edu with SMTP id AA00579 (5.65c/IDA-1.4.4 for ); Mon, 3 Feb 1997 17:19:38 -0600 Received: from [138.23.90.231] by 138.23.90.231 with SMTP; Mon, 3 Feb 1997 15:16:40 -0800 (PST) X-Sender: xhuang@pop.ucr.edu Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 3 Feb 1997 15:19:45 -0800 To: nema-l@unl.edu From: xhuang@ucrac1.ucr.edu (Xiang Huang, UCR Nematology) Subject: Looking for glass beads Dear Colleagues, I am looking for glass beads of diameters of 0.1, 0.2, 0.4, 0.8, and 1.6mm, repectively. Unfortunately they are not available on any catalogs I could check. I really appreciate it if you could provide any information. Thanks in advance. Xiang ------------------------------------------------------- Xiang Huang * Department of Nematology * Tel.: 909/787-4586 University of California * FAX: 909/787-3719 Riverside, CA 92521-0415 * xhuang@ucrac1.ucr.edu ------------------------------------------------------- >From John.Jaenike@jj.biology.rochester.edu Thu Feb 6 06:10:34 1997 Received: from galileo.cc.rochester.edu by crcnis1.unl.edu with SMTP id AA26867 (5.65c/IDA-1.4.4 for ); Thu, 6 Feb 1997 09:54:45 -0600 Received: from jj.biology.rochester.edu. (jj.biology.rochester.edu [128.151.242.36]) by galileo.cc.rochester.edu (8.8.5/8.6.4) with SMTP id KAA20295 for ; Thu, 6 Feb 1997 10:52:01 -0500 (EST) Date: Thu, 06 Feb 1997 11:10:34 EST From: John Jaenike Subject: Nematode gene sequences To: nema-l@unl.edu X-Mailer: LeeMail 2.0.6 Message-Id: Our lab would like to examine intraspecific sequence variation in any single- copy nuclear gene from the insect-parasitic nematode Howardula aoronymphium (Allantonematidae: Tylenchida). Our best bet for designing PCR primers would be to try genes whose sequences are highly conserved across all nematodes. Any suggestions? John Jaenike Department of Biology University of Rochester Rochester, NY 14627 Phone: 716-275-7835 FAX: 716-275-2070 Email: jaenike@jj.biology.rochester.edu >From jjones@scri.sari.ac.uk Thu Feb 6 16:51:18 1997 Received: from caird.scri.sari.ac.uk by crcnis1.unl.edu with SMTP id AA00348 (5.65c/IDA-1.4.4 for ); Thu, 6 Feb 1997 10:53:47 -0600 Received: from law.scri.sari.ac.uk (law.scri.sari.ac.uk [134.36.144.25]) by caird.scri.sari.ac.uk (8.6.9/8.6.9) with ESMTP id QAA18957 for ; Thu, 6 Feb 1997 16:50:46 GMT Received: from LAW/MERCURY by law.scri.sari.ac.uk (Mercury 1.21); 6 Feb 97 16:51:39 GMT Received: from MERCURY by LAW (Mercury 1.21); 6 Feb 97 16:51:21 GMT From: "John Jones" To: nema-l@unl.edu Date: Thu, 6 Feb 1997 16:51:18 GMT Subject: Re: Nematode gene sequences Priority: normal X-Mailer: Pegasus Mail for Windows (v2.42a) Message-Id: <3037820390A@law.scri.sari.ac.uk> > Our lab would like to examine intraspecific sequence variation in any single- > copy nuclear gene from the insect-parasitic nematode Howardula aoronymphium > (Allantonematidae: Tylenchida). Our best bet for designing PCR primers would > be to try genes whose sequences are highly conserved across all nematodes. Any > suggestions? > Dear Dr jaenike, You probably wont get too much intraspecific variation in the coding region of a single copy nuclear gene. How about looking at an intron? It wouldnt be too difficult to design primers that span an intron in, say, a collagen gene. The coding region will be conserved enough to make sure you get amplification from all your samples and I would imagine that the intron sequence would be more likely to show variation. I wouldn't swear to this though - maybe someone else has better suggestions... John ================================================== John Jones Nematology Dept SCRI Invergowrie Dundee DD2 5DA 01382 562731 jjones@scri.sari.ac.uk ==================================================